1.1.1.81: hydroxypyruvate reductase

This is an abbreviated version, for detailed information about hydroxypyruvate reductase, go to the full flat file.

Reaction

D-glycerate
+
NAD(P)+
=
Hydroxypyruvate
+
NAD(P)H
+
H+

Synonyms

At1g12550, AtHPR1, beta-hydroxypyruvate reductase, D-2-hydroxy-acid dehydrogenase, D-glycerate dehydrogenase, glyoxylate reductase/hydroxypyruvate reductase, glyoxylate/hydroxypyruvate reductase, GLYR1, GRHPR, HPR1, HPR2, HPR3, HRP, hydroxypyruvate reductase, More, NADH-dependent hydroxypyruvate reducing enzyme activity, NADH-dependent hydroxypyruvate reductase, NADH-HPR, NADH:hydroxypyruvate reductase, NADPH-dependent hydroxypyruvate reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.81 hydroxypyruvate reductase

Crystallization

Crystallization on EC 1.1.1.81 - hydroxypyruvate reductase

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified detagged recombinant enzyme in ternary complex with product D-glycerate and cofactor NADPH, sitting drop vapour diffusion method, 5.5 mg/ml protein in 20 mM Tris-HCl, pH 8.5, 1 mM 2-mercaptoethanol, 0.2 mM NADPH, and 0.5 mm di-sodium oxalate, mixed with mother liquor, containing 15% w/v PEG 8000, 0.2 M ammonium sulfate, and 0.1 M sodium cacodylate, pH 6.5, to 0.002 ml drops, 18°C, X-ray diffraction structure determination and analysis at 2.2 A resolution
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl
sitting drop vapor diffusion method in the presence of NAD, crystal structure analysis reveals tightly bound NADP(H) at the enzyme originating from Escherichia coli expression, which is not replaceable by NAD
-
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl