1.1.1.69: gluconate 5-dehydrogenase

This is an abbreviated version, for detailed information about gluconate 5-dehydrogenase, go to the full flat file.

Reaction

D-gluconate
+
NAD(P)+
=
5-Dehydro-D-gluconate
+
NAD(P)H
+
H+

Synonyms

5-keto D-gluconate reductase, 5-keto-D-gluconate 5-reductase, 5-keto-D-gluconate reductase, 5-ketogluconate 5-reductase, 5-ketogluconate reductase, 5KGA reductase, 5KGR, dehydrogenase, gluconate 5-, GA5DH, gluconate 5-dehydrogenase, gluconate-5-dehydrogenase, gluconate:NADP 5-oxidoreductase, GNO, GOX2187 gene product, pyrroloquinoline quinone-dependent gluconate-5-dehydrogenase, QGA-5-DH, reductase, 5-ketogluconate 5-

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.69 gluconate 5-dehydrogenase

Cloned

Cloned on EC 1.1.1.69 - gluconate 5-dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as fusion protein with a N-terminal His6 tag in Escherichia coli strain BL21 (DE3)
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3), expression levels vary from colony to colony, cells showing high enzyme expression tend to show less growth, expression system appears to be unstable as expression level decreases when subcultured for several times, best expression results with freshly transformed cells
-
expression in Escherichia coli and Gluconobacter oxydans
-