1.1.1.6: glycerol dehydrogenase

This is an abbreviated version, for detailed information about glycerol dehydrogenase, go to the full flat file.

Reaction

glycerol
+
NAD+
=
Glycerone
+
NADH
+
H+

Synonyms

CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, GldA, GLDH , GlhA, glycerin dehydrogenase, glycerol dehydrogenase, glycerol NAD 2-oxidoreductase, glycerol:NAD+ 2-oxidoreductase, GlyDH, Gro dehydrogenase, GroDHase, gycerol dehydrogenase, NAD+-dependent glycerol dehydrogenase, NAD-linked glycerol dehydrogenase, NAD-specific glycerol dehydrogenase, TmGlyDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.6 glycerol dehydrogenase

Reference

Reference on EC 1.1.1.6 - glycerol dehydrogenase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Altaras, N.E.; Cameron, D.C.
Metabolic engineering of a 1,2-propanediol pathway in Escherichia coli
Appl. Environ. Microbiol.
65
1180-1185
1999
Escherichia coli
Manually annotated by BRENDA team
Kong, Y.C.; May, J.W.; Marshall, J.H.
Glycerol oxidation and triose reduction by pyridine nucleotide-linked enzyme in the fission yeast Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1571-1579
1985
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972h-
-
Manually annotated by BRENDA team
Marshall, J.H.; May, J.W.; Sloan, J.
Purification and properties of glycerol:NAD+ 2-oxidoreductase (glycerol dehydrogenase) from Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1581-1588
1985
Schizosaccharomyces pombe
-
Manually annotated by BRENDA team
Gärtner, G.; Kopperschläger, G.
Purification and properties of glycerol dehydrogenase from Candida valida
J. Gen. Microbiol.
130
3225-3233
1984
Pichia membranifaciens, Pichia membranifaciens H 112
-
Manually annotated by BRENDA team
Nishise, H.; Nagao, A.; Tani, Y.; Yamada, H.
Further characterization of glycerol dehydrogenase from Cellulomonas sp. NT3060
Agric. Biol. Chem.
48
1603-1609
1984
Cellulomonas sp., Cellulomonas sp. NT3060
-
Manually annotated by BRENDA team
Scharschmidt, M.; Pfleiderer, G.; Metz, H.; Brummer, W.
Isolierung und Charakterisierung von Glycerol-Dehydrogenase aus Bacillus megaterium
Hoppe-Seyler's Z. Physiol. Chem.
364
911-921
1983
Bacillus megaterium
-
Manually annotated by BRENDA team
Sasaki, I.; Itoh, N.; Goto, H.; Yamamoto, R.; Tanaka, H.; Yamshita, K.; Yamashita J.; Horio, T.
Specific affinity of glycerol dehydrogenase from Geotrichum candidium for 10-carboxydecyl-sepharose: its application to chromatography for purification of the enzyme
J. Biochem.
91
211-217
1982
Geotrichum candidum
Manually annotated by BRENDA team
Yamada H.; Nagao, A.; Nishise, H.; Tani, Y.
Glycerol dehydrogenase from Cellulomonas sp. NT 3060: purification and characterization
Agric. Biol. Chem.
46
2333-2339
1982
Cellulomonas sp., Cellulomonas sp. NT3060
-
Manually annotated by BRENDA team
Yamada, H.; Nagao, A.; Tani, Y.
Crystallisation of glycerol dehydrogenase
Agric. Biol. Chem.
44
471-472
1980
Cellulomonas sp.
-
Manually annotated by BRENDA team
Ruch, F.E.; Lin, E.C.C.; Kowit, J.D.; Tang, C.T.; Goldberg, A.L.
In vivo inactivation of glycerol dehydrogenase in Klebsiella aerogenes: properties of active and inactivated proteins
J. Bacteriol.
141
1077-1085
1980
Klebsiella aerogenes 2103, Klebsiella aerogenes
Manually annotated by BRENDA team
McGregor, W.G.; Philips, J.; Suelter, C.H.
Purification and kinetic characterization of a monovalent cation-activated glycerol dehydrogenase from Aerobacter aerogenes
J. Biol. Chem.
249
3132-3139
1974
Klebsiella aerogenes 1033, Klebsiella aerogenes
Manually annotated by BRENDA team
Lin, E.C.C.; Magasanik, B.
The activation of glycerol dehydrogenase from Aerobacter aerogenes by monovalent cations
J. Biol. Chem.
235
1820-1823
1960
Klebsiella aerogenes, Klebsiella aerogenes P14
Manually annotated by BRENDA team
Burton, R.M.
Glycerol dehydrogenase from Aerobacter aerogenes
Methods Enzymol.
1
397-400
1955
Klebsiella aerogenes
-
Manually annotated by BRENDA team
Asnis, R.A.; Brodie, A.F.
A glycerol dehydrogenase from Escherichia coli
J. Biol. Chem.
203
153-159
1953
Escherichia coli, Escherichia coli ECFS
Manually annotated by BRENDA team
Nishise, H.; Maehashi, S.; Yamada, H.; Tani, Y.
Effects of organic solvents and chemical modification on the activity of glycerol dehydrogenase
Agric. Biol. Chem.
51
3347-3353
1987
Cellulomonas sp., Cellulomonas sp. NT3060
-
Manually annotated by BRENDA team
Malaoui, H.; Marczak, R.
Separation and characterization of the 1,3-propanediol and glycerol dehydrogenase activities from Clostridium butyricum E5 wild-type and mutant D
J. Appl. Microbiol.
90
1006-1014
2001
Clostridium butyricum, Clostridium butyricum E5
Manually annotated by BRENDA team
Leichus, B.N.; Blanchard, J.S.
Isotopic analysis of the reaction catalyzed by glycerol dehydrogenase
Biochemistry
33
14642-14649
1994
Cellulomonas sp.
Manually annotated by BRENDA team
Liyanage, H.; Kashket, S.; Young, M.; Kashket, E.R.
Clostridium beijerinckii and Clostridium difficile detoxify methylglyoxal by a novel mechanism involving glycerol dehydrogenase
Appl. Environ. Microbiol.
67
2004-2010
2001
Clostridioides difficile, Clostridium beijerinckii
Manually annotated by BRENDA team
Burke, J.; Ruzheinikov, S.N.; Sedelnikova, S.; Baker, P.J.; Holmes, D.; Muir, N.M.; Gore, M.G.; Rice, D.W.
Purification, crystallisation and quaternary structure analysis of a glycerol dehydrogenase S305C mutant from Bacillus stearothermophilus
Acta Crystallogr. Sect. D
57
165-167
2001
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Yamada-Onodera, K.; Yamamoto, H.; Emoto, E.; Kawahara, N.; Tani, Y.
Characterisation of glycerol dehydrogenase from a methylotrophic yeast, Hansenula polymorpha Dl-1, and its gene cloning
Acta Biotechnol.
22
337-353
2002
Ogataea angusta, Ogataea angusta DL-1
-
Manually annotated by BRENDA team
Yamada-Onodera, K.; Yamamoto, H.; Kawahara, N.; Tani, Y.
Expression of the gene of glycerol dehydrogenase from Hansenula polymorpha Dl-1 in Escherichia coli for the production of chiral compounds
Acta Biotechnol.
22
355-362
2002
Ogataea angusta, Ogataea angusta DL-1
-
Manually annotated by BRENDA team
Srinivasan, V.; Ma, K.; Adams, M.W.; Newton, M.G.; Rose, J.P.; Wang, B.C.
Towards the crystal structure of glycerol dehydrogenase from Thermotoga maritima
Acta Crystallogr. Sect. D
58
867-869
2002
Thermotoga maritima
Manually annotated by BRENDA team
Stibor, M.; Potocky, M.; Pickova, A.; Karasova, P.; Russell, N.J.; Kralova, B.
Characterization of cold-active dehydrogenases for secondary alcohols and glycerol in psychrotolerant bacteria isolated from Antarctic soil
Enzyme Microb. Technol.
32
532-538
2003
Arthrobacter sp.
-
Manually annotated by BRENDA team
Pandey, A.; Iyengar, L.
Chemical modification of specific active site amino acid residues of Enterobacter aerogenes glycerol dehydrogenase
J. Enzyme Inhib. Med. Chem.
17
49-53
2002
Klebsiella aerogenes
Manually annotated by BRENDA team
Brinen, L.S.; Canaves, J.M.; Dai, X.; Deacon, A.M.; Elsliger, M.A.; Eshaghi, S.; Floyd, R.; Godzik, A.; Grittini, C.; Grzechnik, S.K.; Guda, C.; Jaroszewski, L.; Karlak, C.; Klock, H.E.; Koesema, E.; Kovarik, J.S.; Kreusch, A.; Kuhn, P.; Lesley, S.A.; McMullan, D.; McPhillips, T.M.; Miller, M.A.; Miller, M.D.; Morse, A.; Moy, K.; Ouyang, J.; Robb, A.; Rodrigues, K.; Selby, T.L.; Spraggon, G.; Stevens, R.C.; Van den Bedem, H.; Velasquez, J.; Vincent, J.; Wang, X.; West, B.; Wolf, G.; Taylor, S.S.; Hodgson, K.O.; Wooley, J.; Wilson, I.A.
Crystal structure of a zinc-containing glycerol dehydrogenase from Thermotoga maritima at 1.5 ANG resolution
Proteins
50
371-374
2003
Thermotoga maritima, Thermotoga maritima TM0423
Manually annotated by BRENDA team
Ichinose, H.; Kamiya, N.; Goto, M.
Enzymatic redox cofactor regeneration in organic media: functionalization and application of glycerol dehydrogenase and soluble transhydrogenase in reverse micelles
Biotechnol. Prog.
21
1192-1197
2005
Escherichia coli, Escherichia coli JM109
Manually annotated by BRENDA team
Katrlik, J.; Mastihuba, V.; Vostiar, I.; Sefcovicova, J.; Stefuca, V.; Gemeiner, P.
Amperometric biosensors based on two different enzyme systems and their use for glycerol determination in samples from biotechnological fermentation process
Anal. Chim. Acta
566
11-18
2006
Cellulomonas sp.
-
Manually annotated by BRENDA team
Oliveira, H.M.; Segundo, M.A.; Lima, J.L.; Grassi, V.; Zagatto, E.A.
Kinetic enzymatic determination of glycerol in wine and beer using a sequential injection system with spectrophotometric detection
J. Agric. Food Chem.
54
4136-4140
2006
Cellulomonas sp., Klebsiella aerogenes
Manually annotated by BRENDA team
Nakayama, S.; Watanabe, H.
Mechanism of hilA repression by 1,2-propanediol consists of two distinct pathways, one dependent on and the other independent of catabolic production of propionate, in Salmonella enterica serovar Typhimurium
J. Bacteriol.
188
3121-3125
2006
Salmonella enterica
Manually annotated by BRENDA team
Lee, W.; DaSilva, N.A.
Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast
Metab. Eng.
8
58-65
2006
Escherichia coli
Manually annotated by BRENDA team
Gamella, M.; Campuzano, S.; Reviejo, A.J.; Pingarron, J.M.
Integrated multienzyme electrochemical biosensors for the determination of glycerol in wines
Anal. Chim. Acta
609
201-209
2008
Cellulomonas sp.
Manually annotated by BRENDA team
Gaetgens, C.; Degner, U.; Bringer-Meyer, S.; Herrmann, U.
Biotransformation of glycerol to dihydroxyacetone by recombinant Gluconobacter oxydans DSM 2343
Appl. Microbiol. Biotechnol.
76
553-559
2007
Gluconobacter oxydans
Manually annotated by BRENDA team
Goncalves, A.T.; Marcal, D.; Carrondo, M.A.; Enguita, F.J.
Crystallization and preliminary X-ray characterization of a glycerol dehydrogenase from the human pathogen Salmonella enterica serovar Typhimurium
Acta Crystallogr. Sect. F
65
698-701
2009
Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZKM9), Salmonella enterica subsp. enterica serovar Typhimurium
Manually annotated by BRENDA team
Dominguez, K.B.; Toth, I.V.; Souto, M.R.; Mendes, F.; Garcia De Maria, C.; Vasconcelos, I.; Rangel, A.O.
Sequential Injection Kinetic Flow Assay for Monitoring Glycerol in a Sugar Fermentation Process by Saccharomyces cerevisiae
Appl. Biochem. Biotechnol.
160
1664-1673
2009
Cellulomonas sp.
Manually annotated by BRENDA team
Hatayama, K.; Yagishita, T.
Regulation of glycerol metabolism in Enterobacter aerogenes NBRC12010 under electrochemical conditions
Appl. Microbiol. Biotechnol.
83
749-756
2009
Klebsiella aerogenes, Klebsiella aerogenes NBRC12010
Manually annotated by BRENDA team
Zhao, L.; Zheng, Y.; Ma, X.; Wei, D.
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Bioprocess Biosyst. Eng.
32
313-320
2009
Klebsiella pneumoniae (A0MLR7), Klebsiella pneumoniae, Klebsiella pneumoniae KG1
Manually annotated by BRENDA team
Durnin, G.; Clomburg, J.; Yeates, Z.; Alvarez, P.J.; Zygourakis, K.; Campbell, P.; Gonzalez, R.
Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli
Biotechnol. Bioeng.
103
148-161
2009
Escherichia coli
Manually annotated by BRENDA team
Mouri, T.; Shimizu, T.; Kamiya, N.; Goto, M.; Ichinose, H.
Design of a cytochrome P450BM3 reaction system linked by two-step cofactor regeneration catalyzed by a soluble transhydrogenase and glycerol dehydrogenase
Biotechnol. Prog.
25
1372-1378
2009
Escherichia coli, Escherichia coli JM109
Manually annotated by BRENDA team
Zhao, L.; Ma, X.; Zheng, Y.; Zhang, J.; Wei, G.; Wei, D.
Over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase in Klebsiella pneumoniae and their effects on conversion of glycerol into 1,3-propanediol in resting cell system
J. Chem. Technol. Biotechnol.
84
626-632
2009
Klebsiella pneumoniae (A0MLR7), Klebsiella pneumoniae KG1 (A0MLR7)
-
Manually annotated by BRENDA team
Zhang, K.; Zhao, W.D.; Li, Q.; Fang, W.G.; Zhu, L.; Shang, D.S.; Chen, Y.H.
Tentative identification of glycerol dehydrogenase as Escherichia coli K1 virulence factor cglD and its involvement in the pathogenesis of experimental neonatal meningitis
Med. Microbiol. Immunol.
198
195-204
2009
Escherichia coli
Manually annotated by BRENDA team
Gonzalez, R.; Murarka, A.; Dharmadi, Y.; Yazdani, S.S.
A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coli
Metab. Eng.
10
234-245
2008
Escherichia coli K-12
Manually annotated by BRENDA team
Shams Yazdani, S.; Gonzalez, R.
Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products
Metab. Eng.
10
340-351
2008
Escherichia coli K-12
Manually annotated by BRENDA team
Camacho Morales, R.L.; Castellanos, A.D.; Zazueta-Sandoval, R.
Analysis of glycerol dehydrogenase activities present in Mucor circinelloides YR-1
Antonie van Leeuwenhoek
98
437-445
2010
Mucor circinelloides, Mucor circinelloides YR-1
Manually annotated by BRENDA team
Matsuzawa, T.; Ohashi, T.; Hosomi, A.; Tanaka, N.; Tohda, H.; Takegawa, K.
The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe
Appl. Microbiol. Biotechnol.
87
715-727
2010
Schizosaccharomyces pombe (O13702), Schizosaccharomyces pombe, Schizosaccharomyces pombe ARC039 (O13702)
Manually annotated by BRENDA team
Zhang, H.; Lountos, G.T.; Ching, C.B.; Jiang, R.
Engineering of glycerol dehydrogenase for improved activity towards 1, 3-butanediol
Appl. Microbiol. Biotechnol.
88
117-124
2010
Escherichia coli, Klebsiella pneumoniae, Klebsiella pneumoniae MGH78578, Salmonella enterica, Salmonella enterica ATCC 9150
Manually annotated by BRENDA team
Piattoni, C.; Figueroa, C.; Asencion Diez, M.; Parcerisa, I.; Antuna, S.; Comelli, R.; Guerrero, S.; Beccaria, A.; Iglesias, A.
Production and characterization of Escherichia coli glycerol dehydrogenase as a tool for glycerol recycling
Process Biochem.
48
406-412
2013
Escherichia coli
-
Manually annotated by BRENDA team
Yao, S.; Mikkelsen, M.J.
Metabolic engineering to improve ethanol production in Thermoanaerobacter mathranii
Appl. Microbiol. Biotechnol.
88
199-208
2010
Thermotoga maritima (Q9WYQ4)
Manually annotated by BRENDA team
Musille, P.; Ortlund, E.
Structure of glycerol dehydrogenase from Serratia
Acta Crystallogr. Sect. F
70
166-172
2014
Serratia sp.
Manually annotated by BRENDA team
Liu, L.; Zhuge, X.; Shin, H.D.; Chen, R.R.; Li, J.; Du, G.; Chen, J.
Improved production of propionic acid in Propionibacterium jensenii via combinational overexpression of glycerol dehydrogenase and malate dehydrogenase from Klebsiella pneumoniae
Appl. Environ. Microbiol.
81
2256-2264
2015
Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. pneumoniae ATCC 12657
Manually annotated by BRENDA team
Beauchamp, J.; Gross, P.G.; Vieille, C.
Characterization of Thermotoga maritima glycerol dehydrogenase for the enzymatic production of dihydroxyacetone
Appl. Microbiol. Biotechnol.
98
7039-7050
2014
Thermotoga maritima (Q9WYQ4), Thermotoga maritima
Manually annotated by BRENDA team
Mahadevan, A.; Fernando, S.
An improved glycerol biosensor with an Au-FeS-NAD-glycerol-dehydrogenase anode
Biosens. Bioelectron.
92
417-424
2016
Cellulomonas sp.
Manually annotated by BRENDA team
Wang, S.; Wang, J.; Zhou, X.; Guo, Y.; Fang, B.
The improvement of stability, activity, and substrate promiscuity of glycerol dehydrogenase substituted by divalent metal ions
Biotechnol. Bioprocess Eng.
18
796-800
2013
Klebsiella pneumoniae subsp. pneumoniae (A6TGD6), Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 (A6TGD6)
-
Manually annotated by BRENDA team
Zhang, J.; Cui, Z.; Chang, H.; Fan, X.; Zhao, Q.; Wei, W.
Conversion of glycerol to 1,3-dihydroxyacetone by glycerol dehydrogenase co-expressed with an NADH oxidase for cofactor regeneration
Biotechnol. Lett.
38
1559-1564
2016
Escherichia coli
Manually annotated by BRENDA team
Zhang, L.; Xu, Q.; Peng, X.; Xu, B.; Wu, Y.; Yang, Y.; Sun, S.; Hu, K.; Shen, Y.
Cloning, expression and characterization of glycerol dehydrogenase involved in 2,3-butanediol formation in Serratia marcescens H30
J. Ind. Microbiol. Biotechnol.
41
1319-1327
2014
Serratia marcescens, Serratia marcescens H30
Manually annotated by BRENDA team
Wang, L.; Wang, J.; Shi, H.; Gu, H.; Zhang, Y.; Li, X.; Wang, F.
Characterization of glycerol dehydrogenase from Thermoanaerobacterium thermosaccharolyticum DSM 571 and GGG motif identification
J. Microbiol. Biotechnol.
26
1077-1086
2016
Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum DSM 571
Manually annotated by BRENDA team
Fang, B.; Jiang, W.; Zhou, Q.; Wang, S.
Codon-optimized NADH oxidase gene expression and gene fusion with glycerol dehydrogenase for bienzyme system with cofactor regeneration
PLoS ONE
10
e0128412
2015
Klebsiella pneumoniae (B2ZPN8), Klebsiella pneumoniae DSM 2026 (B2ZPN8)
Manually annotated by BRENDA team
Fang, B.; Niu, J.; Ren, H.; Guo, Y.; Wang, S.
Mechanistic study of manganese-substituted glycerol dehydrogenase using a kinetic and thermodynamic analysis
PLoS ONE
9
e99162
2014
Klebsiella pneumoniae (B2ZPN8), Klebsiella pneumoniae
Manually annotated by BRENDA team
Zhang, Y.; Ren, H.; Wang, Y.; Chen, K.; Fang, B.; Wang, S.
Bioinspired immobilization of glycerol dehydrogenase by metal ion-chelated polyethyleneimines as artificial polypeptides
Sci. Rep.
6
24163
2016
Klebsiella pneumoniae (B2ZPN8), Klebsiella pneumoniae DSM 2026 (B2ZPN8)
Manually annotated by BRENDA team