1.1.1.51: 3(or 17)beta-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 3(or 17)beta-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

testosterone
+
NAD(P)+
=
androstenedione
+
NAD(P)H
+
H+

Synonyms

17 beta-HSD3, 17 beta-hydroxysteroid dehydrogenase, 17-beta-HSD, 17-ketoreductase, 17beta-HSD, 17beta-HSD (type 1), 17beta-HSD (type 3), 17beta-HSD type 2, 17beta-HSD type 3, 17beta-HSD type II, 17beta-HSD-3, 17beta-HSD2, 17beta-HSD3, 17beta-HSD5, 17beta-HSD7_2, 17beta-HSOR, 17beta-hydroxy steroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase 2, 17beta-hydroxysteroid dehydrogenase type 3, 17beta-hydroxysteroid dehydrogenases, 17beta-hydroxysteroid dehydrogenases type 2, 17beta-hydroxysteroid oxidoreductase, 17betaHSD2, 3 beta-hydroxysteroid dehydrogenase, 3,17beta-HSD, 3,17beta-hydroxysteroid dehydrogenase, 3-beta HSD, 3beta,17beta-hydroxysteroid dehydrogenase, 3beta-HSD, 3beta-HSD (type 1), 3beta-HSD type-I, 3beta-HSD type-II, 3beta-HSD/isomerase, 3beta-hydroxy steroid dehydrogenase, 3beta-hydroxy-steroid dehydrogenase/DELTA5-DELTA4 isomerase, 3beta-hydroxysteroid dehydrogenase, 3beta-hydroxysteroid dehydrogenase/DELTA5-4-isomerase, 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4-isomerase, 3beta-hydroxysteroid dehydrogenase/isomerase, 3beta-hydroxystroid oxidoreductase, 3beta/17beta-hydroxysteroid dehydrogenase, 3beta17betaHSDH, 3betaHSD, AKR1C3, AKR1C35, aldo keto reductase 1C3, beta-hydroxy steroid dehydrogenase, betahsd, dehydrogenase, 17beta-hydroxy steroid, dehydrogenase, 3beta-hydroxy steroid, dehydrogenase, beta-hydroxy steroid, DELTA5-3beta-HSD, DELTA5-3beta-hydroxysteroid dehydrogenase, form 2 type 7 17beta-hydroxysteroid dehydrogenase, HSD, HSD17B3, LOC101842245, More, pQ3 beta HSD, steroid 17beta-reductase, type 17beta-HSD, type 2 17beta-HSD, type 3 17beta-HSD, type 3 17beta-hydroxysteroid dehydrogenase, type 5 17-beta-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.51 3(or 17)beta-hydroxysteroid dehydrogenase

Cloned

Cloned on EC 1.1.1.51 - 3(or 17)beta-hydroxysteroid dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
AKR1C3, quantitative enzyme expression analysis by real-time PCR, recombinant expression in HEK-293 cells
-
cDNA is functionally expressed as a His-tagged fusion protein in Escherichia coli
-
cloning by in silico blast searches in the Taenia solium genome database and RT-PCR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, transient recombinant expression in HEK-293T cells
-
DNA and amino acid sequence determination and analysis, chromosome metaphase, interphase mapping, i.e. FISH mapping, localization on chromosome 10q11.2, stable expression in HEK-293 cells
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
DNA and amino acid sequence determination and analysis, phylogenetic tree, transient expression in HEK-293 cells
-
DNA and amino acid sequence determination and analysis, phylogenetic tree, transient expression in zf4 cells
expressed in COS-7 cells; expressed in COS-7 cells
expressed in HEK-293 cells by transient transfection
gene 3bhsd, quantitative real-time PCR expression analysis
-
gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
gene betahsd, cloning of PhaR and 3,17beta-HSD together with their promoter domains into plasmids pK18 and pUC19 and coexpression in Escherichia coli strain HB101. A 2509 bp DNA fragment, that contains a putative promoter for the bhsd gene (without the phaR gene), is cloned into plasmid pUC2.5-3. The plasmid is transformed into strain HB101 and induced with testosterone. 3,17beta-HSD expression results at a high level, but cannot be further enhanced by testosterone. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21. Promoter cloning
-
gene betahsd, sequence comparisons, cloning and location of the TSS and promoter (126 bp) of the 3,17beta-HSD gene, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
-
gene HSD17B2, quantitative real-time RT-PCR enzyme expression analysis
-
gene HSD17B3, recombinant expression in HEK-293 cells
-
His6-tagged 17beta-HSD2 overexpressed in Sf9 cells
-
isozyme DNA and amino acid sequence determination and analysis, tissue-specific expression and phylogenetic analysis, expression of tissue-specific isozymes 17beta-HSD1 and 17beta-HSD8 in Escherichia coli and in HEK-293 cells
-
overexpression in HEK-293 cells
-
overexpression of type 3 17beta-HSD in HEK-293 cells
-