1.1.1.49: glucose-6-phosphate dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about glucose-6-phosphate dehydrogenase (NADP+), go to the full flat file.

Reaction

D-glucose 6-phosphate
+
NADP+
=
6-phospho-D-glucono-1,5-lactone
+
NADPH
+
H+

Synonyms

6-phosphoglucose dehydrogenase, D-glucose 6-phosphate dehydrogenase, D-glucose 6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate:NADP oxidoreductase, Entner-Doudoroff enzyme, G-6-PD, G-6-PDH, G-6PD, G6PD, G6PD1, G6PD2, G6PD3, G6PD4, G6PD5, G6PD6, G6PDH, G6PDH-1, G6PDH-2, G6PDH1, G6PDH2, G6PDH3, G6PDH4, G6PDH5, G6PDH6, Glc6PDH, glucose 6-phosphate dehydrogenase, glucose 6-phosphate dehydrogenase (NADP), glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, GPD, KlZWF1, NADP-dependent glucose 6-phophate dehydrogenase, NADP-glucose-6-phosphate dehydrogenase, PfGluPho, VEG11, Vegetative protein 11, Zwischenferment

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.49 glucose-6-phosphate dehydrogenase (NADP+)

Temperature Stability

Temperature Stability on EC 1.1.1.49 - glucose-6-phosphate dehydrogenase (NADP+)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 30
-
activity is increased 2-3fold by heating at 25°C (either at pH 10 in 0.5 M glycine KOH buffer or at pH 5 in 0.05 M sodium citrate buffer) prior to assay, the stimulation is reversed by chilling to 0°C but is not reversed at temperatures between 20°C and 30°C
4
-
inactivation at 4°C is partially reversed at room temperature, especially with added NADP+
25
-
half-life: 15 min
45
-
60 min, the wild-type enzyme is completely stable, the mutant P409R shows 86% reduced thermal stability compared to the wild-type enzyme
47
-
50 min, the enzyme is completely inactivated either in the cell free extract or in the concentrated fraction. The thermodynamic parameters of G6PD thermal inactivation suggests the occurrence of two inactivation events both related to breaking of bonds responsible for the active dimer integrity: one, prevailing at low temperature, likely led to the formation of a less active dimer, while the other, prevailing at high temperature, is responsible for the formation of a totally inactive dimer or monomer
50
-
purified recombinant His-tagged enzyme, inactivation
90
-
half-life: 24 h
95 - 100
-
120 min, almost complete loss in activity
100
-
half-life: 12 h
additional information