1.1.1.49: glucose-6-phosphate dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about glucose-6-phosphate dehydrogenase (NADP+), go to the full flat file.

Reaction

D-glucose 6-phosphate
+
NADP+
=
6-phospho-D-glucono-1,5-lactone
+
NADPH
+
H+

Synonyms

6-phosphoglucose dehydrogenase, D-glucose 6-phosphate dehydrogenase, D-glucose 6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate:NADP oxidoreductase, Entner-Doudoroff enzyme, G-6-PD, G-6-PDH, G-6PD, G6PD, G6PD1, G6PD2, G6PD3, G6PD4, G6PD5, G6PD6, G6PDH, G6PDH-1, G6PDH-2, G6PDH1, G6PDH2, G6PDH3, G6PDH4, G6PDH5, G6PDH6, Glc6PDH, glucose 6-phosphate dehydrogenase, glucose 6-phosphate dehydrogenase (NADP), glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, GPD, KlZWF1, NADP-dependent glucose 6-phophate dehydrogenase, NADP-glucose-6-phosphate dehydrogenase, PfGluPho, VEG11, Vegetative protein 11, Zwischenferment

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.49 glucose-6-phosphate dehydrogenase (NADP+)

Purification

Purification on EC 1.1.1.49 - glucose-6-phosphate dehydrogenase (NADP+)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1189fold by ammonium sulfate precipitation and 2',5'-ADP affinity chromatography
-
2000fold by 2times 2',5'-ADP affinity chromatography and gel filtration
-
58fold from leaves by ammonium sulfate precipitation and DEAE ion exchange chromatography
-
74fold by ammonium sulfate precipitation and DEAE ion exchange chromatography
-
978fold from small intestine, by ammonium sulfate precipitation, dialysis, and DEAE ion exchange and 2',5'-ADP affinity chromatography
-
approximately 100-fold purification; approximately 450fold purification
-
both isoforms A(-) and A(+)
-
by affinity chromatography, at 4C, 5343fold with a yield of 52%
-
by affinity chromatography, to homogeneity
by ammonium sulfate precipitation and affinity chromatography, 1691fold, with a yield of 63%
-
by homogenate precipitation, ammonium sulfate precipitation and ion exchange chromatography, at 4C, 124.8fold, with a yield of 57.6%
-
continuous counter-current purification of glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae cells by liquid-liquid extraction using reverse micelles. A biocompatible reverse micellar system consisting of 0.05 M soybean lecithin (zwitterionic surfactants) in isooctane with hexanol is employed to study the influence of different flow-rates on G6PD purification. The results show that the reverse micellar system is able to remove proteins (impurities) from the cell-free. The enzyme recovery yield varies from 32% to 115% and G6PD purification factor from 1.0 to 2.2, under flow-rates ranging from 1.6:1.6 mL/min to 6.0:6.0 ml/min for both phases (micellar and aqueous phases). The steady hold-up values demonstrate that the mass transfer capability of this extraction apparatus is practically constant. The continuous counter-current purification system employed proves to be useful for purifying glucose-6-phosphate dehydrogenase and for maintaining the enzyme activity
-
G6PDH-1; G6PDH-2
-
glucose-6-phosphate dehydrogenase from commercial Saccharomyces cerevisiae was concentrated by reverse micelles liquid-liquid extraction using soybean lecithin. Five successive cycles of extraction ensured a G6PD purification factor of 5.4. The kinetic and thermodynamic properties either of the concentrated fraction or the cell free extract are investigated. While the Michaelis constant for glucose-6-phosphate is almost independent of the presence of cell debris, the maximum initial activity is about 16% higher in its absence. The extraction seems to slightly improve both the enzyme activity and stability
-
isoenzymes I and II
native and recombinant from Escherichia coli
-
native enzyme 1010fold from liver by 2',5'-ADP affinity chromatography
-
native enzyme 1384fold from kidney cortex by ultracentrifugation, 2',5'-ADP affinity and anion exchange chromatography
-
native enzyme 1600fold from erythrocytes by ammonium sulfate fractionation, and 2',5'-ADP affinity chromatography to homogeneity
-
native enzyme 230fold to homogeneity by ammonium sulfate fractionation, affinity chromatography, and gel filtration
-
native enzyme 2488fold from hemolysate by ammonium sulfate, dialysis, and 2',5'-ADP affinity chromatography
-
native enzyme 531fold from kidney, co-purification with 6-phosphogluconate dehydrogenase and glutathione reductase in one chromatographic step by 2',5'-ADP affinity chromatography by using different elution buffers, method development and optimization, overview
-
native enzyme 90fold by anion exchange and affinity chromatography
-
native enzyme by blue native PAGE to homogeneity, method optimization, overview
-
native wild-type from blood leukocyte, and recombinant wild-type and mutant P409R from Saccharomyces cerevisiae, by 2',5'-ADP affinity chromatography
-
partial from liver and kidney
-
partial purification of the enzyme from different tissues by ammonium sulfate fractionation and dialysis
-
rapid partial purifiation from root plastids, 17.7fold
-
rapid procedure, 1.58fold by ammonium sulfate precipitation and ADP resin chromatography
-
recombinant His-tagged enzyme 95.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli, to homogeneity by heat precipitation, and nickel affinity chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by 2',5'-ADP affinity chromatography
-
recombinant wild-type and mutant enzymes from G6PD-deficient Escherichia coli strain DF213
-
to homogeneity by a single nickel affinity chromatographic step
two varients G6PD(Plymouth) (G163D) and G6PD(Mahidol) (G163S)
-
wild-type and clinical mutants purified to homogeneity
-
wild-type enzyme, 4.1fold from blood by ammonium sulfate precipitation, dialysis, and 2',5'-ADP affinity chromatography
-