126.96.36.199: D-galactose 1-dehydrogenase
This is an abbreviated version, for detailed information about D-galactose 1-dehydrogenase, go to the full flat file.
beta-galactose dehydrogenase, D-galactose dehydrogenase, D-galactose: NAD+ 1-oxidoreductase, D-galactose:NAD+ oxidoreductase, GADH, GALDH, NAD-dependent D-galactose dehydrogenase
Purification on EC 188.8.131.52 - D-galactose 1-dehydrogenase
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12% (w/v) ammonium sulfate precipitation and 14% (w/v) polyethylene glycol 2000 precipitation at pH 7.5
ammonium sulfate, CM-Sephadex, DEAE-Sephadex, hydroxyapatite, disc electrophoresis
ammonium sulfate, DEAE-Sepharose, FPLC-Superose 12, beta-galactosidase-galactose dehydrogenase fusion enzyme
ammonium sulfate, heat, phosphate precipitation, DEAE-cellulose, hydroxyapatite
aqueous two-phase system composed of 12% (w/v) PEG-6000, 15% (w/v) K2HPO4 with pH 7.5 at 25°C
by affinity chromatography on chimeric mimodye-ligand gel resins, method evaluation
Mono Q column, partially purified enzyme