1.1.1.44: phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

This is an abbreviated version, for detailed information about phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating), go to the full flat file.

Reaction

6-phospho-D-gluconate
+
NADP+
=
D-ribulose 5-phosphate
+
CO2
+
NADPH
+
H+

Synonyms

6-GPD, 6-P-gluconate dehydrogenase, 6-Pgd, 6-PGDH, 6-PGDHase, 6-phospho-D-gluconate dehydrogenase, 6-phospho-D-gluconate-NADP+ oxidoreductase, decarboxylating, 6-phosphogluconate dehydrogenase, 6-phosphogluconate dehydrogenase (decarboxylating), 6-phosphogluconate dehydrogenase Gnd1, 6-phosphogluconate dehydrogenase, decarboxylating, 6-phosphogluconate-dehydrogenase, 6-phosphogluconate:NADP oxidoreductase, 6-phosphogluconic carboxylase, 6-phosphogluconic dehydrogenase, 6-phosphonogluconate dehydrogenase, 6PDH, 6PG DH, 6PGD, 6PGDH, 6PGDH/Gnd1, D-gluconate-6-phosphate dehydrogenase, gnd, GND1, GNTZII, LlPDH, Os6PGDH2, phosphogluconic acid dehydrogenase, YpjI

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

Storage Stability

Storage Stability on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, stable to freezing and thawing, incubated for 24 h retains at least 70% of its original activity
-
20C, in phosphate buffer pH 6.8 in presence of the reducing agent beta-mercaptoethanol enzyme remains fully active for 24 h
-
4C, 50 mM triethanolamine and 2 mM beta-mercaptoethanol at pH 7.5, 1 year, no loss of activity
-
4C, purified enzyme in 50% glycerol buffer remains active for at least 3 months
-
4C, purified enzyme, stored in 20% glycerol is stable for months
-
4C, retains full activity for several months when stored as an ammonium sulfate suspension or in acetate buffer, pH 6 in presence of a crystal of thymol to prevent bacterial growth
-
4C, stable for 5 h, loses 20% of its activity after 24%, no activity after 3 days
-
4C, stable for several days, as an (NH4)2SO4 suspension stable for several months without any loss of enzyme activity
-
4C, the isoenzyme with an isolectric point of 6.5 retains virtually all its activity after storing for 30 days, the isoenzyme with isoelectric point 6.3 loses about 90% activity after storage for 3 days
-
5C, purified enzyme is stable to storage in 50 mM Tris HCl buffer, pH 7.5, 10 mM dithiothreitol and 20% glycerol, no significant decrease in activity occurs over a 2 week period
-
5C, purified enzyme is stable up to 3 months if care is taken to exclude molecular oxygen from the enzyme solution
-