1.1.1.44: phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

This is an abbreviated version, for detailed information about phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating), go to the full flat file.

Reaction

6-phospho-D-gluconate
+
NADP+
=
D-ribulose 5-phosphate
+
CO2
+
NADPH
+
H+

Synonyms

6-GPD, 6-P-gluconate dehydrogenase, 6-Pgd, 6-PGDH, 6-PGDHase, 6-phospho-D-gluconate dehydrogenase, 6-phospho-D-gluconate-NADP+ oxidoreductase, decarboxylating, 6-phosphogluconate dehydrogenase, 6-phosphogluconate dehydrogenase (decarboxylating), 6-phosphogluconate dehydrogenase Gnd1, 6-phosphogluconate dehydrogenase, decarboxylating, 6-phosphogluconate-dehydrogenase, 6-phosphogluconate:NADP oxidoreductase, 6-phosphogluconic carboxylase, 6-phosphogluconic dehydrogenase, 6-phosphonogluconate dehydrogenase, 6PDH, 6PG DH, 6PGD, 6PGDH, 6PGDH/Gnd1, D-gluconate-6-phosphate dehydrogenase, gnd, GND1, GNTZII, LlPDH, Os6PGDH2, phosphogluconic acid dehydrogenase, YpjI

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

Purification

Purification on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
725.4fold from erythrocytes by ammonium sulfate precipitation, 2',5'-ADP-resin affinity chromatography, and gel filtration
-
78.5fold, partial from erythrocytes by ammonium sulfate precipitation, fraction 60-80% saturation
-
ammonium sulfate precipitation, 2',5'-ADP Sepharose 4B affinity chromatography and Sephadex G200 gel filtration
-
HisTrap column chromatography and Superdex 200 gel filtration
-
native enzyme by partially 27fold ion exchange, affinity and size exclusion chromatography, His-tagged recombinant wild-type and mutant enzymes by nickel affinity chromatography
Ni-NTA column chromatography and ADP-agarose column chromatography
-
Ni-NTA resin chromatography and ADP-agarose affinity column chromatography
-
Ni-NTA resin column chromatography and 2?5?ADP agarose chromatography
-
Ni2+-NTA affinity chromatography
-
Ni2+-NTA column chromatography
partially, fractionation
-
purified from an overexpressing Escherichia coli strain
-
recombinant enzyme
-
recombinant from Escherichia coli
Sephadex G-25 column chromatography, DEAE-Sepharose Fast Flow ion exchange chromatography and 2',5'-ADP Sepharose 4B column chromatography
-
wild-type, mutant col-3, mutant col-10
-