1.1.1.44: phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

This is an abbreviated version, for detailed information about phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating), go to the full flat file.

Reaction

6-phospho-D-gluconate
+
NADP+
=
D-ribulose 5-phosphate
+
CO2
+
NADPH
+
H+

Synonyms

6-GPD, 6-P-gluconate dehydrogenase, 6-Pgd, 6-PGDH, 6-PGDHase, 6-phospho-D-gluconate dehydrogenase, 6-phospho-D-gluconate-NADP+ oxidoreductase, decarboxylating, 6-phosphogluconate dehydrogenase, 6-phosphogluconate dehydrogenase (decarboxylating), 6-phosphogluconate dehydrogenase Gnd1, 6-phosphogluconate dehydrogenase, decarboxylating, 6-phosphogluconate-dehydrogenase, 6-phosphogluconate:NADP oxidoreductase, 6-phosphogluconic carboxylase, 6-phosphogluconic dehydrogenase, 6-phosphonogluconate dehydrogenase, 6PDH, 6PG DH, 6PGD, 6PGDH, 6PGDH/Gnd1, D-gluconate-6-phosphate dehydrogenase, gnd, GND1, GNTZII, LlPDH, Os6PGDH2, phosphogluconic acid dehydrogenase, YpjI

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

General Stability

General Stability on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
a single step of freezing and thawing reduces the enzyme activity by 90%
both isoenzymes stabilized by the H2PO4-
-
denaturation by urea
-
enzyme loses no activity on dialysis overnight against 10 mM EDTA
-
low stability due to absence of inter-subunit salt bridges as compared to the enzyme from Trypanosoma brucei
noteworthy stability to heat and protein denaturants
-
remarkably stable to the action of trypsin, chymotrypsin and elastase
-
stabilized by KCl
-
stable in 1 mM urea
-
stable in 8 M urea, nearly unaffected, only 10% loss of activity after exposure for 60 min
-