1.1.1.43: phosphogluconate 2-dehydrogenase

This is an abbreviated version, for detailed information about phosphogluconate 2-dehydrogenase, go to the full flat file.

Reaction

6-phospho-D-gluconate
+
NAD(P)+
=
6-phospho-2-dehydro-D-gluconate
+
NAD(P)H
+
H+

Synonyms

2-keto-6-phosphogluconate reductase, 6-PGADHase, 6-PGDase, 6-PGDH, 6-phosphogluconate dehydrogenase, 6-phosphogluconate dehydrogenase (NAD), 6-phosphogluconate-dehydrogenase, 6-phosphogluconic dehydrogenase, 6-phosphoglucuronic acid dehydrogenase, 6PGAD, 6PGD, 6PGDH, gluconate 6-phosphate dehydrogenase, phosphogluconate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.43 phosphogluconate 2-dehydrogenase

Purification

Purification on EC 1.1.1.43 - phosphogluconate 2-dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, 2',5'-ADP Sepharose 4B affinity chromatography, and Sephadex G-200 gel filtration
-
ammonium sulfate precipitation, 2’,5’-ADP Sepharose 4B column chromatography, and Sephadex G-25 gel filtration
-
Ni–NTA column chromatography
-
partial, electrophoretic isozyme pattern
purified 2256times in a yield of 44.22% by using ammonium sulfate precipitation and 2', 5'-ADP Sepharose 4B affinity gel. Temperature of +4°C is maintained during the purification process
-