1.1.1.42: isocitrate dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about isocitrate dehydrogenase (NADP+), go to the full flat file.

Reaction

isocitrate
+
NADP+
=
2-oxoglutarate
+
CO2
+
NADPH
+
H+

Synonyms

AfIDH, CgIDH, cICDH, CtIDP1, CtIDP2, cytosolic isocitrate dehydrogenase 1, cytosolic NADP(+)-dependent isocitrate dehydrogenase, cytosolic NADP+-dependent isocitrate dehydrogenase, cytosolic NADP-dependent isocitrate dehydrogenase, cytosolic NADPH-dependent isocitrate dehydrogenase, DpIDH, EcIDH, ICD, ICD1, ICD2, ICDH, ICDH-1, IDH, IDH1, IDH2, IDH3, IDHP, IDP, IDP1, IDP2, IDP3, IDPA, IDPc, IDPm, isocitrate dehydrogenase, isocitrate dehydrogenase (NADP), isocitrate dehydrogenase (NADP-dependent), isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate), isocitrate dehydrogenase 1, isocitrate dehydrogenase 2, isocitrate dehydrogenase-1, isocytrate deyhdrogenase, mICDH, mitochondrial NADP+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase, NADP isocitric dehydrogenase, NADP+-dependent Ds-threo-isocitrate dehydrogenase, NADP+-dependent Ds-threo-isocitrate:NADP+ oxidoreductase, NADP+-dependent IDH, NADP+-dependent isocitrate dehydrogenase, NADP+-ICDH, NADP+-IDH, NADP+-isocitrate dehydrogenase, NADP+-linked isocitrate dehydrogenase, NADP+-specific ICDH, NADP+-specific isocitrate dehydrogenase, NADP-dependent IDH, NADP-dependent isocitrate dehydrogenase, NADP-dependent isocitric dehydrogenase, NADP-ICDH, NADP-IDH, NADP-IDH Idp1p, NADP-isocitrate dehydrogenase, NADP-linked isocitrate dehydrogenase, NADP-specific isocitrate dehydrogenase, NADPH-dependent isocitrate dehydrogenase, oxalosuccinate decarboxylase, oxalsuccinic decarboxylase, perICDH, PS-NADP-IDH, TaIDH, TM1148

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.42 isocitrate dehydrogenase (NADP+)

Temperature Stability

Temperature Stability on EC 1.1.1.42 - isocitrate dehydrogenase (NADP+)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
-
isoenzyme II
20
-
isoenzyme II
35
-
isoenzyme C, half-life 20 min
38 - 50
about 50% of IDH activity is preserved at 38°C after 20 min of incubation, the enzyme is inactive at 50°C
47
-
purified enzyme, 20 min, loss of 50% activity
49
-
isoenzyme A, half-life 20 min
52
-
isoenzyme C, half-life 20 min, presence of isocitrate
58
-
isoenzyme A, T50-value, presence of isocitrate
69
-
isoenzyme B, T50-value, presence of isocitrate
70
-
t1/2: 106 min
70 - 80
-
stable
74
-
10 min, 50% loss of activity, mutant enzyme A336F
75
half-life: 24 min
82
melting temperature at pH 7.5 is 82.2°C
85
melting temperature at pH 5.8 is 84.5°C
89
-
10 min, 50% loss of activity, mutant enzyme I321L
90
-
half life of 22 min in 50 mM tricine-KOH buffer, pH 8
91
-
10 min, 50% loss of activity, mutant enzyme A325P/G326S
98
-
the Tm-value is 98.3°C
110
-
the Tm-value is 109.9°C
additional information