1.1.1.42: isocitrate dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about isocitrate dehydrogenase (NADP+), go to the full flat file.

Reaction

isocitrate
+
NADP+
=
2-oxoglutarate
+
CO2
+
NADPH
+
H+

Synonyms

AfIDH, CgIDH, cICDH, CtIDP1, CtIDP2, cytosolic isocitrate dehydrogenase 1, cytosolic NADP(+)-dependent isocitrate dehydrogenase, cytosolic NADP+-dependent isocitrate dehydrogenase, cytosolic NADP-dependent isocitrate dehydrogenase, cytosolic NADPH-dependent isocitrate dehydrogenase, DpIDH, EcIDH, ICD, ICD1, ICD2, ICDH, ICDH-1, IDH, IDH1, IDH2, IDH3, IDHP, IDP, IDP1, IDP2, IDP3, IDPA, IDPc, IDPm, isocitrate dehydrogenase, isocitrate dehydrogenase (NADP), isocitrate dehydrogenase (NADP-dependent), isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate), isocitrate dehydrogenase 1, isocitrate dehydrogenase 2, isocitrate dehydrogenase-1, isocytrate deyhdrogenase, mICDH, mitochondrial NADP+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase, NADP isocitric dehydrogenase, NADP+-dependent Ds-threo-isocitrate dehydrogenase, NADP+-dependent Ds-threo-isocitrate:NADP+ oxidoreductase, NADP+-dependent IDH, NADP+-dependent isocitrate dehydrogenase, NADP+-ICDH, NADP+-IDH, NADP+-isocitrate dehydrogenase, NADP+-linked isocitrate dehydrogenase, NADP+-specific ICDH, NADP+-specific isocitrate dehydrogenase, NADP-dependent IDH, NADP-dependent isocitrate dehydrogenase, NADP-dependent isocitric dehydrogenase, NADP-ICDH, NADP-IDH, NADP-IDH Idp1p, NADP-isocitrate dehydrogenase, NADP-linked isocitrate dehydrogenase, NADP-specific isocitrate dehydrogenase, NADPH-dependent isocitrate dehydrogenase, oxalosuccinate decarboxylase, oxalsuccinic decarboxylase, perICDH, PS-NADP-IDH, TaIDH, TM1148

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.42 isocitrate dehydrogenase (NADP+)

General Stability

General Stability on EC 1.1.1.42 - isocitrate dehydrogenase (NADP+)

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
3 M NaCl stabilizes the recombinant enzyme
Q8X277
enzyme stability is mainly sensitive to cations and very little or not sensitive to anions. Divalent cations induce a strong shift of the active/inactive transition towards low salt concentration. Deactivation due to incubation of the enzyme at low NaCl concentrations is related to irreversible dissociation of the active dimeric species towards a monomer, which can be described as an extended inactive monomeric species
Q8X277
instable in solutions of low ionic strength, stabilization by ammonium sulfate
low molecular weight form of Acinetobacter calcoaceticus enzyme more heat stable than high molecular weight form
-
Mg2+ stabilizes the enzyme during purification
-
stabilization by dithiothreitol
-
the inter-domain ionic network might be responsible for additional stabilization through a significant kinetic barrier in the unfolding pathway that can explain the larger difference observed between the folding and unfolding transitions of the wild type comparted to the mutant enzyme R211M
-
with a residual amount of secondary structure
Q8X277