1.1.1.42: isocitrate dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about isocitrate dehydrogenase (NADP+), go to the full flat file.

Reaction

isocitrate
+
NADP+
=
2-oxoglutarate
+
CO2
+
NADPH
+
H+

Synonyms

AfIDH, CgIDH, cICDH, CtIDP1, CtIDP2, cytosolic isocitrate dehydrogenase 1, cytosolic NADP(+)-dependent isocitrate dehydrogenase, cytosolic NADP+-dependent isocitrate dehydrogenase, cytosolic NADP-dependent isocitrate dehydrogenase, cytosolic NADPH-dependent isocitrate dehydrogenase, DpIDH, EcIDH, ICD, ICD1, ICD2, ICDH, ICDH-1, IDH, IDH1, IDH2, IDH3, IDHP, IDP, IDP1, IDP2, IDP3, IDPA, IDPc, IDPm, isocitrate dehydrogenase, isocitrate dehydrogenase (NADP), isocitrate dehydrogenase (NADP-dependent), isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate), isocitrate dehydrogenase 1, isocitrate dehydrogenase 2, isocitrate dehydrogenase-1, isocytrate deyhdrogenase, mICDH, mitochondrial NADP+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase, NADP isocitric dehydrogenase, NADP+-dependent Ds-threo-isocitrate dehydrogenase, NADP+-dependent Ds-threo-isocitrate:NADP+ oxidoreductase, NADP+-dependent IDH, NADP+-dependent isocitrate dehydrogenase, NADP+-ICDH, NADP+-IDH, NADP+-isocitrate dehydrogenase, NADP+-linked isocitrate dehydrogenase, NADP+-specific ICDH, NADP+-specific isocitrate dehydrogenase, NADP-dependent IDH, NADP-dependent isocitrate dehydrogenase, NADP-dependent isocitric dehydrogenase, NADP-ICDH, NADP-IDH, NADP-IDH Idp1p, NADP-isocitrate dehydrogenase, NADP-linked isocitrate dehydrogenase, NADP-specific isocitrate dehydrogenase, NADPH-dependent isocitrate dehydrogenase, oxalosuccinate decarboxylase, oxalsuccinic decarboxylase, perICDH, PS-NADP-IDH, TaIDH, TM1148

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.42 isocitrate dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.42 - isocitrate dehydrogenase (NADP+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 polymorphic isozymes IDHP-A and IDHP-B, isozymes show allelic variants in populations belonging to different living heights in the stream, analysis of allelic frequencies, overview
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construction of a genomic cDNA library, gene Cl-idh, DNA sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
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DNA and amino acid sequence determination and analysis, expression as Strep-tagged protein in Escherichia coli BLR(DE3) exclusively in inclusion bodies, removal of 27 amino acids from the N-terminus results in expression of partially soluble protein
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exchange of promotors and altering of organellar targeting for expression of isozymes IDO2 and IDO3 in mitochondria, and of isozymes IDP1 and IDP3 in the cytosol, functional complementation studies using mutant strains
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expressed in Escherichia coli
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expressed in Escherichia coli BL21
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli strain MV1190
expressed in HeLa cells
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expressed in Hep-G2 cells
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expression in Escherichia coli
expression in Escherichia coli as maltose binding fusion protein, wild-type and selenomethionine enzyme variants
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expression in Escherichia coli or Saccharomyces cerevisiae; expression of mouse isoenzyme IDP2 in Escherichia coli or Saccharomyces cerevisiae. Mouse enzyme can compensate for loss of yeast cytosolic IDP2 and of peroxisomal IDP3IDP3. Removal of the peroxisomal targeting signal of the mouse enzyme precludes both localization in peroxisomes and compensation for loss of yeast IDP3
expression of C-terminally His-tagged enzyme in Escherichia coli BL21(DE3)
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expression of His-tagged IDPA in Escherichia coli strain BL21(DE3), expression of IDPA inm Saccharomyces cerevisiae, it behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequenceand occurs in cytosol and peroxisomes, complementation study in Saccharomyces cerevisiae disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, and IDPA lacking the mitochondrial targeting sequence and containing a different PTS1, which results in the same expression level and subcellular orientation, subcellular localization study, overview
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expression of His-tagged mutant enzymes C269S and C379S in Escherichia coli
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expression of isozyme IDP1 and IDP2 as His-tagged enzymes in a disruption mutant
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expression of myc-tagged wild-type IDH1 and R132H mutant IDH1 in U-87MG glioblastoma cells
expression of the cDNA in a construct of nucleotides 1-1714 with rat PEPCK promotor and the SV-40 polyadenylation signal, permanant expression in 3T3-L1 cells and in transgenic mice, the latter are constructed by infection of fertilized eggs
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expression of the gene in Escherichia coli by connecting it with the T7 promoter
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expression of the IDH1R132H mutant at a level similar to the endogenous protein in the cytoplasm of glioblastoma U-87MG cells causes a dose-dependent reduction of 2-oxoglutarate levels. Overexpression of the IDH1R132H mutant in U-87MG cells stimulates expression of HIF-1alpha target genes. Overexpression of wild-type IDH1 reduces HIF-1alpha protein levels in HeLa and U-87MG cells. Expression of FLAG-tagged wild-type and R132 mutants IDH1 in HEK-293T cells and of His-tagged enzymes in Escherichia coli
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expression of wild-type and mutant enzymes in Escherichia coli
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expression of wild-type and mutant enzymes in Escherichia coli as maltose binding fusion proteins
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expression of wild-type and mutant enzymes in Escherichia coli as maltose-binding fusion proteins
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expression of wild-type and mutant enzymes in Escherichia coli TB1 as maltose binding fusion proteins
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gene icd, sequence comparisons, expression of His6-tagged in Escherichia coli strain Rosetta (DE3)
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gene idh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha
gene idh1, expression of Myc-tagged wild-type and Myc-tagged mutant enzymes in HEK-293T cells
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gene NADP-ICDH, DNA and amino acid sequence determination and analysis, phylogenetic tree
gene of mitochondrial isozyme, DNA and amino acid sequence determination and analysis, subcellular RT-PCR analysis of brain cells
gene Rv3339c, expression of N-terminally His6-tagged enzyme
gene Tc00.1047053506925.319 or IDH2, recombinant expression of the N-terminally His6-tagged enzyme in Escherichia coli; gene Tc00.1047053511575.60 or IDH1, recombinant expression of the N-terminally His6-tagged enzyme in Escherichia coli, co-expression with bacterial GroEL/GroES chaperones is required for IDH1 in order to achieve a soluble enzyme
genotyping in diverse cancer cell lines, overview
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IDCH gene, DNA and amino acid sequence determination by combinated PCR and screening of genomic lambda EMBL3 library, and sequence analysis, overexpression in Escherichia coli BL21(DE3) as insoluble protein in inclusion bodies
Q8X277
IDH1, genotyping and genomic profile, overview
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IDH1, genotyping in acute myelogenous leukemia patients, overview; IDH2, genotyping in acute myelogenous leukemia patients, overview
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isozyme cICDH is encoded by a single gene
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NADP+-ICDH contains two different translational ATG start sites, fusion of mitochondrial and chloroplastic targeting signals to YFP reporter, and transient expression in Nicotiana tabacum using Agrobacterium tumefaciens transfection method. The chloroplast-tageting signal constructs are localizes in the chloroplast, while the mitochondrial constructs are localized in either in mitochondria and chloroplasts, due to an 50 amino acid additional part that is added in expression in cultivars Xanthi and Petit Havana
SdIDH, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)
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wild-type and mutant enzymes are expressed in Escherichia coli
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