1.1.1.41: isocitrate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about isocitrate dehydrogenase (NAD+), go to the full flat file.

Reaction

isocitrate
+
NAD+
=
2-oxoglutarate
+
CO2
+
NADH

Synonyms

At2g17130, At4g35260, At4g35650, At5g03290, AtIDH I, AtIDH II, AtIDH V, AtIDH VI, beta-decarboxylating dehydrogenase, beta-ketoglutaric-isocitric carboxylase, CaIDH, CcIDH, hNAD-IDHalpha, homodimeric NAD+-linked isocitrate dehydrogenase, Icd-2, ICDH, ICDH1, IDH, IDH I, IDH II, IDH III, IDH IV, IDH V, IDH-I, IDH-II, IDH-V, IDH1, IDH2, IDH3A, IDH3alpha, IDH5, IDHa, isocitrate dehydrogenase, isocitrate dehydrogenase 2, isocitrate dehydrogenase 3alpha subunit, isocitrate-homoisocitrate dehydrogenase, isocitric acid dehydrogenase, isocitric dehydrogenase, McIDH, mIDH, MiIDH, mitochondrial NAD-dependent isocitrate dehydrogenase, monomeric NAD-IDH, NAD dependent isocitrate dehydrogenase, NAD isocitrate dehydrogenase, NAD isocitric dehydrogenase, NAD(P)+-dependent isocitrate dehydrogenase, NAD+-dependent ICDH, NAD+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase 1, NAD+-dependent isocitrate dehydrogenase 3, NAD+-IDH, NAD+-specific ICDH, NAD+-specific IDH, NAD+-specific isocitrate dehydrogenase, NAD-Dependent IDH, NAD-dependent isocitrate dehydrogenase, NAD-ICDH, NAD-IDH, NAD-isocitrate dehydrogenase, NAD-linked isocitrate dehydrogenase, NAD-specific isocitrate dehydrogenase, OlIDH, OtIDH, PF0202, PH1722, Rv0066c, threo-DS-isocitrate:NAD+ oxidoreductase (decarboxylating), type II homodimeric NAD-IDH, type II NAD+-specific isocitrate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.41 isocitrate dehydrogenase (NAD+)

Temperature Stability

Temperature Stability on EC 1.1.1.41 - isocitrate dehydrogenase (NAD+)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
full loss in activity in a few hours
33
purified recombinant enzyme, 20 min, loss of 50% activity
38
purified recombinant enzyme, 20 min, loss of over 90% activity
40 - 55
recombinant CaIDH retains the majority of the activity below 45C, its activity drops rapidly as the temperature is raised. Incubation at 55C for 20 min causes a 55% or 75% loss of activity in the presence of Mg2+ or Mn2+, respectively
42
-
10 min, 50% loss in activity
45 - 50
recombinant OlIDH is stable below 45C, but its activity rapidly declines as the temperature is raised. Incubation at 45C for 20 min causes a 28% or 21% loss of activity in the presence of Mg2+ or Mn2+, respectively, whereas incubation at 50C causes a 91% or 84% loss of activity in the presence of Mg2+ or Mn2+, respectively
50
-
purified recombinant enzyme, inactivation
102
-
the apparent melting temperature is 102.3C, mutant enzyme D328K
103
-
the apparent melting temperature is 102.9C, mutant enzyme D328K/I329Y
additional information
-
thermal denaturation is an irreversible process