1.1.1.41: isocitrate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about isocitrate dehydrogenase (NAD+), go to the full flat file.

Reaction

isocitrate
+
NAD+
=
2-oxoglutarate
+
CO2
+
NADH

Synonyms

At2g17130, At4g35260, At4g35650, At5g03290, AtIDH I, AtIDH II, AtIDH V, AtIDH VI, beta-decarboxylating dehydrogenase, beta-ketoglutaric-isocitric carboxylase, Icd-2, ICDH, ICDH1, IDH, IDH I, IDH II, IDH III, IDH IV, IDH V, IDH-I, IDH-II, IDH-V, IDH1, IDH2, IDH5, IDHa, isocitrate dehydrogenase, isocitrate dehydrogenase 2, isocitrate-homoisocitrate dehydrogenase, isocitric acid dehydrogenase, isocitric dehydrogenase, McIDH, mIDH, mitochondrial NAD-dependent isocitrate dehydrogenase, NAD dependent isocitrate dehydrogenase, NAD isocitrate dehydrogenase, NAD isocitric dehydrogenase, NAD(P)+-dependent isocitrate dehydrogenase, NAD+-dependent ICDH, NAD+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase 1, NAD+-specific ICDH, NAD+-specific IDH, NAD+-specific isocitrate dehydrogenase, NAD-Dependent IDH, NAD-dependent isocitrate dehydrogenase, NAD-ICDH, NAD-IDH, NAD-isocitrate dehydrogenase, NAD-linked isocitrate dehydrogenase, NAD-specific isocitrate dehydrogenase, PF0202, PH1722, Rv0066c, threo-DS-isocitrate:NAD+ oxidoreductase (decarboxylating)

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.41 isocitrate dehydrogenase (NAD+)

Purification

Purification on EC 1.1.1.41 - isocitrate dehydrogenase (NAD+)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration
-
ammonium sulfate fractionation and gel filtration
-
gel filtration
-
Hi-Trap chelating column chromatography and Superdex 200 gel filtration
-
Hi-Trap chelating HP column chromatography
-
mitochondrial preparation
-
multi-step purification of the native enzyme, 520fold to homogeneity, partial purification of the recombinant enzyme from Escherichia coli
Ni-NTA column chromatography
-
Ni2+-affinity chromatography
Ni2+-nitrilotriacetate (Ni2+-NTA) column chromatography
Ni2+-nitrilotriacetic acid resin chromatography
-
Ni2+-nitrilotriacetic acid superflow resin chromatography
-
partially by mitochondrion isolation in a Percoll gradient, and ammonium sulfate precipitation in the 60-80% fraction, DEAE cellulose chromatography, and desalting
-
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from strain IDH12DELTAL by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3)
-
recombinant wild-type and mutant enzymes from Escherichia coli
recombinant wild-type and mutant NAD-IDHs from Escherichia coli by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration
-