1.1.1.41: isocitrate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about isocitrate dehydrogenase (NAD+), go to the full flat file.

Reaction

isocitrate
+
NAD+
=
2-oxoglutarate
+
CO2
+
NADH

Synonyms

At2g17130, At4g35260, At4g35650, At5g03290, AtIDH I, AtIDH II, AtIDH V, AtIDH VI, beta-decarboxylating dehydrogenase, beta-ketoglutaric-isocitric carboxylase, Icd-2, ICDH, ICDH1, IDH, IDH I, IDH II, IDH III, IDH IV, IDH V, IDH-I, IDH-II, IDH-V, IDH1, IDH2, IDH5, IDHa, isocitrate dehydrogenase, isocitrate dehydrogenase 2, isocitrate-homoisocitrate dehydrogenase, isocitric acid dehydrogenase, isocitric dehydrogenase, McIDH, mIDH, mitochondrial NAD-dependent isocitrate dehydrogenase, NAD dependent isocitrate dehydrogenase, NAD isocitrate dehydrogenase, NAD isocitric dehydrogenase, NAD(P)+-dependent isocitrate dehydrogenase, NAD+-dependent ICDH, NAD+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase 1, NAD+-specific ICDH, NAD+-specific IDH, NAD+-specific isocitrate dehydrogenase, NAD-Dependent IDH, NAD-dependent isocitrate dehydrogenase, NAD-ICDH, NAD-IDH, NAD-isocitrate dehydrogenase, NAD-linked isocitrate dehydrogenase, NAD-specific isocitrate dehydrogenase, PF0202, PH1722, Rv0066c, threo-DS-isocitrate:NAD+ oxidoreductase (decarboxylating)

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.41 isocitrate dehydrogenase (NAD+)

Cloned

Cloned on EC 1.1.1.41 - isocitrate dehydrogenase (NAD+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned and overexpressed in Escherichia coli
-
DNA and amino acid sequence determination and analysis, genotyping and sequence comparison with the acient ancestor teosinte, Zea mays ssp. Parviglumis, overview
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)RP cells
-
expressed in Escherichia coli BL21-Gold (DE3) cells
-
expressed in Escherichia coli strain BL21-CodonPlus (DE3)-RIL
-
expressed in Saccharomyces cerevisiae
expression in Escherichia coli
-
expression of His-tagged wild-type and mutant enzymes in yeast strain IDH12DELTAL deficient in both subunits of the enzyme
-
expression of wild-type and mutant NAD-IDHs in Escherichia coli
-
gene icd, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain EB106
gene idh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
gene idh, expression of wild-type and mutant enzymes in Escherichia coli glutamate auxotrophic strain Dicd::kanr fused to gene icd, the endogenous icd gene is replaced by the kanamycin resistance gene, the wild-type phenotype of the icd defective strain on minimal medium without glutamate is restored
-
gene RtIDH1, DNA and amino acid sequence determination and analysis, sequence comparison, expression in the Saccharomyces cerevisiae strain BY4741 idhDELTA mutant, RtIdh expression leads to increased intracellular lipid content and extracellular citrate concentration with increasing carbon/nitrogen molar ratio of the media under nitrogen limiting conditions; gene RtIDH2, DNA and amino acid sequence determination and analysis, sequence comparison, expression in the Saccharomyces cerevisiae strain BY4741 idhDELTA mutant, RtIdh expression leads to increased intracellular lipid content and extracellular citrate concentration with increasing carbon/nitrogen molar ratio of the media under nitrogen limiting conditions
gene SlIDH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in antisense orientation in tomato plants
-
genotyping in diverse cancer cell lines, overview
-
His-tagged enzyme expression in Escherichia coli strain BL21-Gold(DE3)
-
overexpression of the wild-type and a mutant enzyme, the latter containing the wild-type 2 subunits alpha and beta plus an additional third one, gamma, overexpression of the 3-subunit mutant and the same mutant with point mutations of Arg-residues in Escherichia coli
-
wild-type and mutant enzymes are expressed in a idh1DELTAidh2DELTA yeast strain which contains deletion/insertion mutations in genomic IDH1 and IDH2 loci; wild-type and mutant enzymes are expressed in a idh1DELTAidh2DELTA yeast strain which contains deletion/insertion mutations in genomic IDH1 and IDH2 loci