1.1.1.40: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

This is an abbreviated version, for detailed information about malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+), go to the full flat file.

Reaction

(S)-malate
+
NADP+
=
pyruvate
+
CO2
+
NADPH

Synonyms

ADP-malic enzyme2, c-NADP-ME, C4 NADP-malic enzyme, C4 photosynthetic NADP-malic enzyme, C4-NADP-malic enzyme, C4-NADP-ME, ChlME1, ChlME2, cytoNADPME, cytosolic malic enzyme, cytosolic NADP+-dependent isoform, cytosolic NADP+-dependent malic enzyme, L-malate: NADP oxidoreductase (decarboxylating), L-malate: NADP oxidoreductase [OAA decarboxylating], L-malate: NADP oxidoreductase [oxaloacetate decarboxylating], L-malate:NADP oxidoreductase, L-malate:NADP oxidoreductase (oxaloacetate decarboxylating), MaeB, MalA, malate dehydrogenase (decarboxylating, NADP), malate dehydrogenase (NADP, decarboxylating), malE1, malic enzyme, malic enzyme 1, malic enzyme 3, malic enzyme-NADP, ME, ME-NADP, ME1, ME2, ME3, NAD(P)+-malic enzyme, NADP dependent malic enzyme, NADP malic enzyme, NADP+ dependent malic enzyme, NADP+-dependent malic enzyme, NADP+-dependent malic enzyme 3, NADP+-ME, NADP-dependent malate dehydrogenase, NADP-dependent malic enzyme, NADP-linked decarboxylating malic enzyme, NADP-malate enzyme, NADP-malic enzyme, NADP-malic enzyme 2, NADP-MDH, NADP-ME, NADP-ME1, NADP-ME2, NADP-ME3, NADP-ME4, NADP-specific malate dehydrogenase, NADP-specific malic enzyme, NADP-specific ME, pyruvic-malic carboxylase, TME

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

Purification

Purification on EC 1.1.1.40 - malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 distinct enzyme forms
-
331fold to homogeneity by a 4-step chromatographical procedure
5160fold from oil seed
-
isozyme 2, partially from leaves, 513fold
-
native enzyme by anion exchange and affinity chromatography
-
Ni-NTA column chromatography
Ni-NTA-Sepharose column chromatography
-
nickel affinity column chromatography
-
nickel affinity gel column chromatography
-
nickel-containing His-bind column chromatography
-
partial
recombinant enzyme from Escherichia coli by pecipitation with streptomycin sulfate, DEAE ion exchange, affinity and gel filtration
-
recombinant fusion protein; recombinant fusion protein
recombinant His-tagged chimeric NADP-ME mutants from Escherichia coli strain BL21(DE3) by two different steps of affinity chromatography to homogeneity
-
recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged isozyme Hvme1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, native enzyme partially by chloroplast isolation; recombinant His-tagged isozyme Hvme3 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged MaeB from strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged mutant enzyme to homogeneity from Escherichia coli by metal affinity chromatography
-
recombinant isozymes PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5 from Escherichia coli by glutathione affinity chromatography
-
recombinant ME1 2229fold from INS-1 832/13 cell cytosolic fractions by 2',5'-ADP affinity chromatography
-
recombinant mutant enzymes
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 by anion exchange chromatography and 2',5'-ADP affinity chromatography to over 95% purity
-
recombinant wild-type and mutants from Escherichia coli to homogeneity
-
separation of isozymes in native PAGE
-