1.1.1.40: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

This is an abbreviated version, for detailed information about malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+), go to the full flat file.

Reaction

(S)-malate
+
NADP+
=
pyruvate
+
CO2
+
NADPH

Synonyms

ADP-malic enzyme2, c-NADP-ME, C4 NADP-malic enzyme, C4 photosynthetic NADP-malic enzyme, C4-NADP-malic enzyme, C4-NADP-ME, ChlME1, ChlME2, cytoNADPME, cytosolic malic enzyme, cytosolic NADP+-dependent isoform, cytosolic NADP+-dependent malic enzyme, L-malate: NADP oxidoreductase (decarboxylating), L-malate: NADP oxidoreductase [OAA decarboxylating], L-malate: NADP oxidoreductase [oxaloacetate decarboxylating], L-malate:NADP oxidoreductase, L-malate:NADP oxidoreductase (oxaloacetate decarboxylating), MaeB, MalA, malate dehydrogenase (decarboxylating, NADP), malate dehydrogenase (NADP, decarboxylating), malE1, malic enzyme, malic enzyme 1, malic enzyme 3, malic enzyme-NADP, ME, ME-NADP, ME1, ME2, ME3, NAD(P)+-malic enzyme, NADP dependent malic enzyme, NADP malic enzyme, NADP+ dependent malic enzyme, NADP+-dependent malic enzyme, NADP+-dependent malic enzyme 3, NADP+-ME, NADP-dependent malate dehydrogenase, NADP-dependent malic enzyme, NADP-linked decarboxylating malic enzyme, NADP-malate enzyme, NADP-malic enzyme, NADP-malic enzyme 2, NADP-MDH, NADP-ME, NADP-ME1, NADP-ME2, NADP-ME3, NADP-ME4, NADP-specific malate dehydrogenase, NADP-specific malic enzyme, NADP-specific ME, pyruvic-malic carboxylase, TME

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

Crystallization

Crystallization on EC 1.1.1.40 - malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
10 mg/ml purified enzyme in quarternary complex with NADP+, Mn2+, and oxalate, closed enzyme form, hanging drop vapour diffusion method, 4°C, from 20 mM Tris, pH 7.4, 10 mM oxalate, 5 mM MnCl2, 0.23 mM NADP+, and 2 mM DTT, with a reservoir solution containing 100 mM sodium citrate, pH 5.5, 8% PEG 6000, and 1 M LiCl, 3-4 days, X-ray diffraction structure determination and analysis at 2.5 A resolution, atomic model
-
hanging drop vapor diffusion method, using 250 mM LiCl and 19% (w/v) PEG 3350
recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography
-