1.1.1.40: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

This is an abbreviated version, for detailed information about malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+), go to the full flat file.

Reaction

(S)-malate
+
NADP+
=
pyruvate
+
CO2
+
NADPH

Synonyms

ADP-malic enzyme2, c-NADP-ME, C4 NADP-malic enzyme, C4 photosynthetic NADP-malic enzyme, C4-NADP-malic enzyme, C4-NADP-ME, ChlME1, ChlME2, cytoNADPME, cytosolic malic enzyme, cytosolic NADP+-dependent isoform, cytosolic NADP+-dependent malic enzyme, L-malate: NADP oxidoreductase (decarboxylating), L-malate: NADP oxidoreductase [OAA decarboxylating], L-malate: NADP oxidoreductase [oxaloacetate decarboxylating], L-malate:NADP oxidoreductase, L-malate:NADP oxidoreductase (oxaloacetate decarboxylating), MaeB, MalA, malate dehydrogenase (decarboxylating, NADP), malate dehydrogenase (NADP, decarboxylating), malE1, malic enzyme, malic enzyme 1, malic enzyme 3, malic enzyme-NADP, ME, ME-NADP, ME1, ME2, ME3, NAD(P)+-malic enzyme, NADP dependent malic enzyme, NADP malic enzyme, NADP+ dependent malic enzyme, NADP+-dependent malic enzyme, NADP+-dependent malic enzyme 3, NADP+-ME, NADP-dependent malate dehydrogenase, NADP-dependent malic enzyme, NADP-linked decarboxylating malic enzyme, NADP-malate enzyme, NADP-malic enzyme, NADP-malic enzyme 2, NADP-MDH, NADP-ME, NADP-ME1, NADP-ME2, NADP-ME3, NADP-ME4, NADP-specific malate dehydrogenase, NADP-specific malic enzyme, NADP-specific ME, pyruvic-malic carboxylase, TME

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

Cloned

Cloned on EC 1.1.1.40 - malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3'-UTR region, cloning, sequencing, and analysis
co-expression in strain W3110 with 5-aminolevulinate synthase, EC 2.3.1.37, from Rhodobacter sphaeroides, hemA gene, for reconstruction of the 5-aminolevulinic acid biosynthesis, subcloning in strain DH5alpha, increased C4 metabolism via NADP-dependent malic enzyme expression results in increased 5-aminolevulinate production by 5-aminolevulinate synthase in Escherichia coli
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cytosolic and mitochondrial isozymes, expression in of His-tagged isozymes ME1 and ME2 Escherichia coli Rosetta (DE3)pLysS; expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3)
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cytosolic and mitochondrial isozymes, expression of His-tagged ME2 in Escherichia coli Rosetta (DE3)pLysS and of His-tagged ME1 in Escherichia coli BL(DE3); expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3)
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DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain JM109
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expressed in Chlorella pyrenoidosa
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expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta cells
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expressed in Mucor circinelloides strain MU290
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expressed in Rhodotorula glutinis strain GM4; ME gene, DNA and amino acid sequence determination and analysis, recombinant enzyme expression of the enzyme in Rhodotorula glutinis strain GM4 using the PGK1 promoter, a strong constitutive promoter of yeast PGK1 gene, co-expression with the PGK1 gene from Saccharomyces cerevisiae
expression in Arabidopsis thaliana under control of the 35S promoter
expression in Escherichia coli
-
expression in Escherichia coli TH-2 cells as a fusion protein including a 15-residue N-terminal leader from beta-galactosidase coded by the lacZ gene
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expression in Escherichia coli, NADP-ME1; expression in Escherichia coli, NADP-ME2; expression in Escherichia coli, NADP-ME3; expression in Escherichia coli, NADP-ME4
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expression in tobacco plants via infection with Agrobacterium tumefaciens
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expression of a glutathione S-transferase fusion proteins NADP-ME2 in Escherichia coli; expression of a glutathione S-transferase fusion proteins NADP-ME3 in Escherichia coli
expression of His-tagged chimeric NADP-ME mutants in Escherichia coli strain BL21(DE3)
-
expression of isozyme NADP-ME2 in Escherichia coli strain BL21(DE3)
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expression of the maize enzyme in Arabidopsis thaliana under control of the cauliflower mosaic virus 35S promoter
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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expression of wild-type and mutant isozymes NADP-ME2 in Escherichia coli
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expression of wild-type and mutants in Escherichia coli BL21(DE3)
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five NADP-ME genes PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, individual expression of GST-tagged isozymes in Escherichia coli
-
functional expression of ME1, under the control of cytomegalovirus, which also directs the transcription of GFP from an internal ribosome entry site, in Rattus norvegicus INS-1 832/13 cells and in Mus musculus islets
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gene hvme1, DNA and amino acid sequence determintion and analysis, isozyme expression analysis, hvme1 promoter analysis, expression of His-tagged isozyme Hvme1 in Escherichia coli strain BL21(DE3); gene hvme2, DNA and amino acid sequence determintion and analysis, isozyme expression analysis, hvme1 promoter analysis; gene hvme3, DNA and amino acid sequence determintion and analysis, isozyme expression analysis, expression of His-tagged isozyme Hvme3 in Escherichia coli strain BL21(DE3)
gene maeB, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene maeB, overexpression in wild-type strain W3110, and in strains deficient for pyruvate formate lyase, lactate dehydrogenase, and glucose phosphotransferase, overview
-
gene malE, DNA and amino acid sequence determination and analysis, genomic library construction, screening, and functional enzyme expression in Escherichia coli
gene Sco5261, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3)
gene tme, subcloning in Escherichia coli, expression in bacteroids deficient for the NAD+-dependent malic enzyme encoded by gene dme, EC 1.1.1.39
-
genes NADP-ME1, NADP-ME2, and NADP-ME3, quantitative RT-PCR expression analysis
-
isozyme DNA and amino acid sequence determination and analysis, phylogenetic analysis, overview
isozyme NADP-ME2, DNA and amino acid sequence determination and analysis by screening of a root cDNA library, expressionin transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens strain EHA105-mediated transformation
isozyme NADP-ME2, expression in Escherichia coli; nadp-me1, expression in Escherichia coli
isozymes expression analysis, overview
-
ME1 and ME3, quantitative real time PCR expression analysis
-
mutant enzymes expressed in Escherichia coli
-
NADP-MDH gene, cDNA library screening, promoter analysis and DNA and amino acid sequence determination and analysis, genetic structure and sequence comparison with Brassicaceae plant sequences, overview, expression oof NADP-MDH-HIS3 fusion genes in the yeast two-hybrid system, Saccharomyces cerevisiae strain YM4271, identifying interaction with DNA binding proteins, overview
overexpression in Escherichia coli
-
overexpression of His-tagged wild-type and mutants in Escherichia coli strain DH5alpha, functional complementation of the enzyme deficient Escherichia coli triple mutant strain EJ1321
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preparation of a genomic library, DNA and amino acid sequence determination and analysis, detailed phylogenetic reconstruction of malic enzymes, overview
subcloning in strain K-12, expression of the His-tagged enzyme in strain BL21(DE3)
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