1.1.1.4: (R,R)-butanediol dehydrogenase
This is an abbreviated version!
For detailed information about (R,R)-butanediol dehydrogenase, go to the full flat file.
Reaction
+
=
+
+
Synonyms
(2R,3R)-2,3-BDH, (2R,3R)-2,3-butanediol dehydrogenase, (R)-2,3-butanediol dehydrogenase, (R)-diacetyl reductase, (R,R)-butane-2,3-diol dehydrogenase, 1-amino-2-propanol dehydrogenase, 1-amino-2-propanol oxidoreductase, 2,3-BDH/AR, 2,3-butanediol dehydrogenase, 2,3-butanediol dehydrogenase/acetoin reductase, 2R,3R-2,3-BD dehydrogenase, 2R-3R-BDH, acetoin reductase, acetoin/diacetyl reductase, ADH-9, aminopropanol oxidoreductase, AR/BDH, BaBdhA, BcBDH, BDH, BDH1, Bdh1p, BDH2, Bdh2p, BDH99::67, BdhA, BtBDH, ButA, butanediol dehydrogenase, ButB, butylene glycol dehydrogenase, butyleneglycol dehydrogenase, D-(-)-butanediol dehydrogenase, D-1-amino-2-propanol dehydrogenase, D-1-amino-2-propanol:NAD+ oxidoreductase, D-2,3-BD dehydrogenase, D-aminopropanol dehydrogenase, dehydrogenase, D-aminopropanol, dehydrogenase, D-butanediol, DHAD, diacetyl reductase (acetoin), EC 1.1.1.74, GDH, GldA, meso-2,3-butanediol dehydrogenase, meso-BDH, MF996569, More, NAD(H)-dependent 2,3-butanediol dehydrogenase, NADH-dependent 2,3-butanediol dehydrogenase, PA4153, PB24_3312, R,R-2,3-butanediol dehydrogenase/meso-2,3-butanediol dehydrogenase/diacetyl reductase, R,R-BD-DH, R,R-BDH, R-selective 2,3-butanediol dehydrogenase, SLAC
ECTree
KCat KM Value
KCat KM Value on EC 1.1.1.4 - (R,R)-butanediol dehydrogenase
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0.033
(2R,3R)-2,3-butanediol
pH 7.5, 25°C
0.03 - 7.379
(2R,3R)-butane-2,3-diol
221.2
(2S)-acetoin
-
at pH 10.5 and 30°C
187.5
(2S,3S)-butane-2,3-diol
-
at pH 10.5 and 30°C
0.31 - 2.51
(3R,3S)-butane-2,3-diol
0.9
(R,R)-butane-2,3-diol
-
at pH 10.5 and 30°C
61.1
diacetyl
at pH 10.0 and 45°C
0.32 - 5.7
glycolaldehyde
0.13 - 3.11
meso-butane-2,3-diol
9.875
NAD+
with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C
71.63
NADH
with diacetyl as cosubstrate, at pH 10.0 and 45°C
0.03
(2R,3R)-butane-2,3-diol
-
enzyme DhaD, at pH 9.0 and 30°C
0.03
(2R,3R)-butane-2,3-diol
-
enzyme GldA, at pH 9.0 and 30°C
7.379
(2R,3R)-butane-2,3-diol
at pH 6.5 and 55°C
0.31
(3R,3S)-butane-2,3-diol
-
enzyme DhaD, at pH 9.0 and 30°C
2.51
(3R,3S)-butane-2,3-diol
-
enzyme GldA, at pH 9.0 and 30°C
0.35
(R)-acetoin
mutant S199W, pH 7.5, 25°C
0.48
(R)-acetoin
mutant S199G, pH 7.5, 25°C
0.9
(R)-acetoin
mutant S199C, pH 7.5, 25°C
1.58
(R)-acetoin
-
at pH 7.0 and 30°C
1.6
(R)-acetoin
wild-type, pH 7.5, 25°C
1.9
(R)-acetoin
mutant S199A, pH 7.5, 25°C
2.8
(R)-acetoin
mutant S199R, pH 7.5, 25°C
7.8
2-butanone
mutant S199W, pH 7.5, 25°C
15
2-butanone
mutant S199C, pH 7.5, 25°C
17
2-butanone
mutant S199G, pH 7.5, 25°C
21
2-butanone
wild-type, pH 7.5, 25°C
26
2-butanone
mutant S199R, pH 7.5, 25°C
61
2-butanone
mutant S199A, pH 7.5, 25°C
4.79
acetaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y
12.6
acetaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y
14
acetaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B
15.43
acetaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B
17
acetaldehyde
pH 7.5, 25°C
0.04
acetoin
-
cosubstrate NADPH, pH 7.0, temperature not specified in the publication
0.4
acetoin
-
cosubstrate NADH, pH 7.0, temperature not specified in the publication
24
acetone
mutant S199W, pH 7.5, 25°C
30
acetone
mutant S199C, pH 7.5, 25°C
39
acetone
mutant S199G, pH 7.5, 25°C
55
acetone
wild-type, pH 7.5, 25°C
67
acetone
mutant S199A, pH 7.5, 25°C
81
acetone
mutant S199R, pH 7.5, 25°C
0.11
glycerol
-
enzyme DhaD, at pH 9.0 and 30°C
1.3
glycerol
-
enzyme GldA, at pH 9.0 and 30°C
0.32
glycolaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B
0.41
glycolaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B
2.41
glycolaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y
5.7
glycolaldehyde
recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y
0.13
meso-butane-2,3-diol
-
enzyme DhaD, at pH 9.0 and 30°C
3.11
meso-butane-2,3-diol
-
enzyme GldA, at pH 9.0 and 30°C
890
NADPH
mutant S199R, pH 7.5, 25°C
8900
NADPH
mutant S199A, pH 7.5, 25°C
39000
NADPH
wild-type, pH 7.5, 25°C