1.1.1.39: malate dehydrogenase (decarboxylating)

This is an abbreviated version, for detailed information about malate dehydrogenase (decarboxylating), go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
pyruvate
+
CO2
+
NADH

Synonyms

AT2g13560, At4g00570, AZC3656 protein, cytosolic malic enzyme, dehydrogenase, malate, diphosphate nucleotide dependent malic enzyme, DMA, DME, m-NAD-ME, malic enzyme, malic enzyme 2, malic enzyme-NAD, ME, ME-NAD, ME1, ME2, mitochondrial malic enzyme, mitochondrial NAD(P)+-dependent malic enzyme, mitochondrial NAD+-dependent malic enzyme, mitochondrial NAD-malic enzyme, NAD(P)+-malic enzyme, NAD+-dependent malic enzyme, NAD-dependent malic enzyme, NAD-dependent malic enzyme 1, NAD-dependent malic enzyme 2, NAD-malate dehydrogenase, NAD-malic enzyme, NAD-ME, NAD-ME1, NAD-ME2, NAD-MEH, NAD-preferring malic enzyme, NAD-preferring ME, NAD-specific malic enzyme, pyruvic-malic carboxylase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.39 malate dehydrogenase (decarboxylating)

Purification

Purification on EC 1.1.1.39 - malate dehydrogenase (decarboxylating)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 1500fold to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, adsorption chromatography, ultrafiltration, and gel filtration
native enzyme 44fold
-
native enzyme 84.7fold by anion exchange and affinity chromatography, and gel filtration; native NAD-preferring malic enzyme 84.7fold by anion exchange and affinity chromatography followed by gel filtration
-
native enzyme from fresh sperm cells by anion exchange and affinity chromatography and gel filtration to over 99% purity
-
native enzyme partially by preparation of mitochondria, method, overview
-
recombinant His-tagged enzyme AZC3656 from Escherichia coli by nickel affinity chromatography
recombinant His-tagged NAD-ME1 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration; recombinant His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filteration; recombinant His-tagged NAD-MEH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant NAD-ME1, NAD-ME2, and NAD-MEH; recombinant NAD-ME1, NAD-ME2, and NAD-MEH
-
several steps
-