1.1.1.39: malate dehydrogenase (decarboxylating)

This is an abbreviated version, for detailed information about malate dehydrogenase (decarboxylating), go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
pyruvate
+
CO2
+
NADH

Synonyms

AZC3656 protein, dehydrogenase, malate, diphosphate nucleotide dependent malic enzyme, DMA, DME, m-NAD-ME, malic enzyme, malic enzyme 2, malic enzyme-NAD, ME, ME-NAD, ME2, mitochondrial malic enzyme, mitochondrial NAD(P)+-dependent malic enzyme, mitochondrial NAD+-dependent malic enzyme, mitochondrial NAD-malic enzyme, NAD(P)+-malic enzyme, NAD+-dependent malic enzyme, NAD-dependent malic enzyme, NAD-malic enzyme, NAD-ME, NAD-ME1, NAD-ME2, NAD-MEH, NAD-preferring malic enzyme, NAD-preferring ME, NAD-specific malic enzyme, pyruvic-malic carboxylase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.39 malate dehydrogenase (decarboxylating)

Cloned

Cloned on EC 1.1.1.39 - malate dehydrogenase (decarboxylating)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
expression of His-tagged NAD-ME1 and of mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3); expression of His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3); expression of His-tagged NAD-MEH in Escherichia coli strain BL21(DE3)
-
expression of mutant enzymes in Escherichia coli strain M15
-
gene AtNAD-ME1, DNA and amino acid sequence determination and analysis, phylogenetic tree; gene AtNAD-ME2, DNA and amino acid sequence determination and analysis, phylogenetic tree
gene dme or azc3656, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli
gene maeE, is encoded in a cluster consisting of two diverging operons, maePE and maeKR, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
gene Sco2951, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3)
-
isozyme expression analysis, overview
-
ME2, quantitative real time PCR expression analysis
-
recombinant expression of NAD-ME1, NAD-ME2, and NAD-MEH; recombinant expression of NAD-ME1, NAD-ME2, and NAD-MEH
-