1.1.1.376: L-arabinose 1-dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about L-arabinose 1-dehydrogenase [NAD(P)+], go to the full flat file.
Reaction
Synonyms
AraDH, HVO_A0330, HVO_B0032, L-arabino-aldose dehydrogenase, L-arabinose 1-dehydrogenase, L-arabinose/D-galactose 1-dehydrogenase, L-AraDH, NAD(P)-dependent L-arabinose 1-dehydrogenase
ECTree
1.1.1.376 L-arabinose 1-dehydrogenase [NAD(P)+]Advanced search results
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results (Engineering
Engineering on EC 1.1.1.376 - L-arabinose 1-dehydrogenase [NAD(P)+]
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D168A
mutant shows no activity under standard assay conditions
H119N
N172A
kinetic analysis shows that the N172A mutant decreases by 4 orders of magnitude in kcat/Km values, compared with the wild-type enzyme
N173A
N173G
N173Q
N173S
N173V
S37D
7.3fold increase in Km-value for NADP+ and 6.5fold decrease in KM-value for NAD+
S37D/R38A
mutant enzyme shows a complete reversal of coenzyme specificity. The kcat/Km value for NADP+ decreases further by 20fold from that of the S37D mutant, and the ratio of NADP+ to NAD+ is about 0.07
W152A
W152F
W152H
W152Y
W231A
N173A
W152F
-
kcat/KM for L-arabinose is 28.4fold lower than the wild-type value
-
W231A
N172A
-
kinetic analysis shows that the N172A mutant decreases by 4 orders of magnitude in kcat/Km values, compared with the wild-type enzyme
-
H119N
kcat/KM for L-arabinose is 8088fold lower than the wild-type value
H119N
mutant emzyme is significantly active, Km value is markedly higher (4450fold) than that of wild-type
N173A
kcat/KM for L-arabinose is 6111fold lower than the wild-type value
N173G
kcat/KM for L-arabinose is 3427fold lower than the wild-type value
N173Q
kcat/KM for L-arabinose is 9735fold lower than the wild-type value
N173S
kcat/KM for L-arabinose is 7051fold lower than the wild-type value
N173V
kcat/KM for L-arabinose is 1874fold lower than the wild-type value
W152A
kcat/KM for L-arabinose is 106fold lower than the wild-type value
W152A
the kcat/Km value by 2 orders of magnitude from wild-type enzyme
W152F
kcat/KM for L-arabinose is 28.4fold lower than the wild-type value
W152F
mutant shows an 14fold higher Km value than the W152Y mutant
W152H
kcat/KM for L-arabinose is 2.8fold lower than the wild-type value
W152Y
kcat/KM for L-arabinose is 1.7fold lower than the wild-type value
W152Y
mutant shows an 14fold higher Km value than the W152Y mutant
W231A
kcat/KM for L-arabinose is 33.2fold lower than the wild-type value
W231A
the kcat/Km value by 2 orders of magnitude from wild-type enzyme
N173A
-
kcat/KM for L-arabinose is 6111fold lower than the wild-type value
-
W231A
-
kcat/KM for L-arabinose is 33.2fold lower than the wild-type value
-
W231A
-
the kcat/Km value by 2 orders of magnitude from wild-type enzyme
-
