1.1.1.37: malate dehydrogenase

This is an abbreviated version, for detailed information about malate dehydrogenase, go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
oxaloacetate
+
NADH
+
H+

Synonyms

(R)-2-hydroxyacid dehydrogenase, (S)-malate dehydrogenase, CaMDH, cMDH, cMDH -S, cMDH-L, cyMDH, cytosolic malate dehydrogenase, cytosolic MDH, cytosolic NAD-dependent malate dehydrogenase, halophilic malate dehydrogenase, HmMalDH, L-malate dehydrogenase, L-malate-NAD oxidoreductase, L-malate: NAD oxidoreductase, L-malate: NAD+ oxidoreductase, L-malate:NAD-oxidoreductase, L-MDH, m-MDH, malate (NAD) dehydrogenase, malate dehydrogenase, malate dehydrogenase (NAD), malate dehydrogenase 1, malate dehydrogenase 2, malate: NAD oxidoreductase, MalDH, malic acid dehydrogenase, malic dehydrogenase, mbNAD-MDH, MDH, MDH A, MDH B1, MDH B2, Mdh1, MDH2, Mdh2a, Mdh2b, mitochondrial malate dehydrogenase, mitochondrial MDH, mMDH, mNAD-MDH, More, NAD+-dependent malate dehydrogenase, NAD+-dependent MDH, NAD+-MDH enzymes, NAD-dependent malate dehydrogenase, NAD-dependent malic dehydrogenase, NAD-dependent MDH, NAD-L-malate dehydrogenase, NAD-linked malate dehydrogenase, NAD-malate dehydrogenase, NAD-malic dehydrogenase, NAD-MDH, NAD-specific malate dehydrogenase, Pcal_1699, peroxisomal NAD+-malate dehydrogenase 1, peroxisomal NAD+-malate dehydrogenase 2, PMDH, PMDH1, PMDH2, regulatory subunit of nucleic acid-conducting channel, s-MDH, SrMalDH, TaMDH, VEG69, Vegetative protein 69, YlMdh2p, [LDH-like] MDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.37 malate dehydrogenase

Temperature Stability

Temperature Stability on EC 1.1.1.37 - malate dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-10 - 35
-
at MgCl concentrations below 0.5 M or CaCl concentrations below 0.35 M, the stability of the protein increases with decreasing temperature down to -10°C. At higher concentrations (0.8-1.5 M MgCl2 or 0.5-1.0 M CaCl2), the curves are bell-shaped with symmetric decreases in stability around 4°C
5 - 40
gradual decrease in activity above 40°C
5 - 60
purified His-tagged cMDH, high stability within this range
10 - 45
-
the parasite enzyme activity is not affected in a broad temperature range (from 10°C to 45°C), the enzyme is also stable at 20°C and 37°C over a 30 min period
20
stable for at least 30 min
37
stable for at least 30 min
38
-
retains 100% activity after 45 min incubation, only 50% activity remains after 15 min at 45°C
47
-
complete inactivation
50.5
Tm, melting temperature
51
-
m-MDH, activity reduced to 50%
63
-
t1/2 is 510 min for the wild-type enzyme and 15 min for mutant enzyme E243R
68
melting temperature of wild-type enzyme at pH 7.5 is 67.8°C
78.5
purified His-tagged cMDH, high stability within this range
79
melting temperature of wild-type enzyme at pH 6.0 is 79.4°C
86
melting temperature of mutant enzyme E165Q at pH 4.4 is 85.8°C
91
melting temperature of mutant enzyme E165Q at pH 6.0 is 90.6°C, melting temperature of mutant enzyme E165Q at pH 7.5 or mutant enzyme E165K at pH 6.0 is 91.4°C
92
melting temperature of mutant enzyme E165K at pH 7.5 is 91.7°C
98
more than 90% residual activity after 6 h in boiling water. No significant change in CD spectra up to 100°C
101
half-life: more than 80 min. Marked stabilizing effect of 1 M K2HPO4 with an increase in thermostability of approximately 40% at 101°C
102
half-life: 30 min
103
half-life: 18 min
additional information