1.1.1.37: malate dehydrogenase

This is an abbreviated version, for detailed information about malate dehydrogenase, go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
oxaloacetate
+
NADH
+
H+

Synonyms

(R)-2-hydroxyacid dehydrogenase, (S)-malate dehydrogenase, CaMDH, cMDH, cMDH -S, cMDH-L, cyMDH, cytosolic malate dehydrogenase, cytosolic MDH, cytosolic NAD-dependent malate dehydrogenase, halophilic malate dehydrogenase, HmMalDH, L-malate dehydrogenase, L-malate-NAD oxidoreductase, L-malate: NAD oxidoreductase, L-malate: NAD+ oxidoreductase, L-malate:NAD-oxidoreductase, L-MDH, m-MDH, malate (NAD) dehydrogenase, malate dehydrogenase, malate dehydrogenase (NAD), malate dehydrogenase 1, malate dehydrogenase 2, malate: NAD oxidoreductase, MalDH, malic acid dehydrogenase, malic dehydrogenase, mbNAD-MDH, MDH, MDH A, MDH B1, MDH B2, Mdh1, MDH2, Mdh2a, Mdh2b, mitochondrial malate dehydrogenase, mitochondrial MDH, mMDH, mNAD-MDH, More, NAD+-dependent malate dehydrogenase, NAD+-dependent MDH, NAD+-MDH enzymes, NAD-dependent malate dehydrogenase, NAD-dependent malic dehydrogenase, NAD-dependent MDH, NAD-L-malate dehydrogenase, NAD-linked malate dehydrogenase, NAD-malate dehydrogenase, NAD-malic dehydrogenase, NAD-MDH, NAD-specific malate dehydrogenase, Pcal_1699, peroxisomal NAD+-malate dehydrogenase 1, peroxisomal NAD+-malate dehydrogenase 2, PMDH, PMDH1, PMDH2, regulatory subunit of nucleic acid-conducting channel, s-MDH, SrMalDH, TaMDH, VEG69, Vegetative protein 69, YlMdh2p, [LDH-like] MDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.37 malate dehydrogenase

Temperature Stability

Temperature Stability on EC 1.1.1.37 - malate dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-10 - 35
-
at MgCl concentrations below 0.5 M or CaCl concentrations below 0.35 M, the stability of the protein increases with decreasing temperature down to -10C. At higher concentrations (0.8-1.5 M MgCl2 or 0.5-1.0 M CaCl2), the curves are bell-shaped with symmetric decreases in stability around 4C
5 - 40
gradual decrease in activity above 40C
5 - 60
purified His-tagged cMDH, high stability within this range
10 - 45
-
the parasite enzyme activity is not affected in a broad temperature range (from 10C to 45C), the enzyme is also stable at 20C and 37C over a 30 min period
20
stable for at least 30 min
37
stable for at least 30 min
38
-
retains 100% activity after 45 min incubation, only 50% activity remains after 15 min at 45C
47
-
complete inactivation
50.5
Tm, melting temperature
51
-
m-MDH, activity reduced to 50%
63
-
t1/2 is 510 min for the wild-type enzyme and 15 min for mutant enzyme E243R
68
melting temperature of wild-type enzyme at pH 7.5 is 67.8C
78.5
purified His-tagged cMDH, high stability within this range
79
melting temperature of wild-type enzyme at pH 6.0 is 79.4C
86
melting temperature of mutant enzyme E165Q at pH 4.4 is 85.8C
91
melting temperature of mutant enzyme E165Q at pH 6.0 is 90.6C, melting temperature of mutant enzyme E165Q at pH 7.5 or mutant enzyme E165K at pH 6.0 is 91.4C
92
melting temperature of mutant enzyme E165K at pH 7.5 is 91.7C
98
more than 90% residual activity after 6 h in boiling water. No significant change in CD spectra up to 100C
101
half-life: more than 80 min. Marked stabilizing effect of 1 M K2HPO4 with an increase in thermostability of approximately 40% at 101C
102
half-life: 30 min
103
half-life: 18 min
additional information