1.1.1.37: malate dehydrogenase

This is an abbreviated version, for detailed information about malate dehydrogenase, go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
oxaloacetate
+
NADH
+
H+

Synonyms

(R)-2-hydroxyacid dehydrogenase, (S)-malate dehydrogenase, CaMDH, cMDH, cMDH -S, cMDH-L, cyMDH, cytosolic malate dehydrogenase, cytosolic MDH, cytosolic NAD-dependent malate dehydrogenase, halophilic malate dehydrogenase, HmMalDH, L-malate dehydrogenase, L-malate-NAD oxidoreductase, L-malate: NAD oxidoreductase, L-malate: NAD+ oxidoreductase, L-malate:NAD-oxidoreductase, L-MDH, m-MDH, malate (NAD) dehydrogenase, malate dehydrogenase, malate dehydrogenase (NAD), malate dehydrogenase 1, malate dehydrogenase 2, malate: NAD oxidoreductase, MalDH, malic acid dehydrogenase, malic dehydrogenase, mbNAD-MDH, MDH, MDH A, MDH B1, MDH B2, Mdh1, MDH2, Mdh2a, Mdh2b, mitochondrial malate dehydrogenase, mitochondrial MDH, mMDH, mNAD-MDH, More, NAD+-dependent malate dehydrogenase, NAD+-dependent MDH, NAD+-MDH enzymes, NAD-dependent malate dehydrogenase, NAD-dependent malic dehydrogenase, NAD-dependent MDH, NAD-L-malate dehydrogenase, NAD-linked malate dehydrogenase, NAD-malate dehydrogenase, NAD-malic dehydrogenase, NAD-MDH, NAD-specific malate dehydrogenase, Pcal_1699, peroxisomal NAD+-malate dehydrogenase 1, peroxisomal NAD+-malate dehydrogenase 2, PMDH, PMDH1, PMDH2, regulatory subunit of nucleic acid-conducting channel, s-MDH, SrMalDH, TaMDH, VEG69, Vegetative protein 69, YlMdh2p, [LDH-like] MDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.37 malate dehydrogenase

Storage Stability

Storage Stability on EC 1.1.1.37 - malate dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, in 20 mM potassium phosphate buffer, pH 7.0, 0.01% 2-mercaptoethanol/40% glycerol, can be stored for at least 2 months without loss of activity
-
-15°C, in 10 mM potassium phosphate buffer, pH 7.5, containing 0.1 M KCl, stable for at least 3 months with no apparent loss of activity
-
-20°C, activity tends to decay rapidly after purification
-
-20°C, can be stored at least several months without any appreciable loss in activity
-
-20°C, can be stored over months
-
-20°C, completely inactivated
-
-20°C, stable
-
-80°C, remains active for several weeks
-
-80°C, stable for 4 months
-
0°C, purified enzymes are stable for up to 2 months in 0.05 M potassium phosphate buffer, pH 7.5 containing 0.001 M 2-mercaptoethanol
-
20°C, dilute enzyme preparation stable for at least 2 weeks
-
25°C, loses not more than 20% activity within 2 weeks
-
4°C, at pH 8 in 0.05 M Tris/HCl containing 1 M NaCl, several weeks without appreciable loss of activity
-
4°C, no appreciable loss in activity for up to 3 months
-
4°C, pH 7.0-9.0, concentration of 0.0018 mg/ml, quite stable. Below pH 7.0, inactivation occurs
-
4°C, purified enzyme remains stable for up to 1 month
-
4°C, stable for several months under oxic conditions
4°C, storage in Tris-HCl, 0.01 M, pH 8, 0.2 M NaCl results in a 96% loss of activity within 48 h, loss of activity can be prevented completely for at least 4 weeks by storage in the same buffer, containing 1 M NaCl, under the latter conditions the enzyme is inactive, activity is completely restored upon dilution of the salt to 20 mM
-