1.1.1.37: malate dehydrogenase

This is an abbreviated version, for detailed information about malate dehydrogenase, go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
oxaloacetate
+
NADH
+
H+

Synonyms

(R)-2-hydroxyacid dehydrogenase, (S)-malate dehydrogenase, CaMDH, cMDH, cMDH -S, cMDH-L, cyMDH, cytosolic malate dehydrogenase, cytosolic MDH, cytosolic NAD-dependent malate dehydrogenase, halophilic malate dehydrogenase, HmMalDH, L-malate dehydrogenase, L-malate-NAD oxidoreductase, L-malate: NAD oxidoreductase, L-malate: NAD+ oxidoreductase, L-malate:NAD-oxidoreductase, L-MDH, m-MDH, malate (NAD) dehydrogenase, malate dehydrogenase, malate dehydrogenase (NAD), malate dehydrogenase 1, malate dehydrogenase 2, malate: NAD oxidoreductase, MalDH, malic acid dehydrogenase, malic dehydrogenase, mbNAD-MDH, MDH, MDH A, MDH B1, MDH B2, Mdh1, MDH2, Mdh2a, Mdh2b, mitochondrial malate dehydrogenase, mitochondrial MDH, mMDH, mNAD-MDH, More, NAD+-dependent malate dehydrogenase, NAD+-dependent MDH, NAD+-MDH enzymes, NAD-dependent malate dehydrogenase, NAD-dependent malic dehydrogenase, NAD-dependent MDH, NAD-L-malate dehydrogenase, NAD-linked malate dehydrogenase, NAD-malate dehydrogenase, NAD-malic dehydrogenase, NAD-MDH, NAD-specific malate dehydrogenase, Pcal_1699, peroxisomal NAD+-malate dehydrogenase 1, peroxisomal NAD+-malate dehydrogenase 2, PMDH, PMDH1, PMDH2, regulatory subunit of nucleic acid-conducting channel, s-MDH, SrMalDH, TaMDH, VEG69, Vegetative protein 69, YlMdh2p, [LDH-like] MDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.37 malate dehydrogenase

Cloned

Cloned on EC 1.1.1.37 - malate dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA encoding mitochondrial malate dehydrogenase sequenced
-
cloned with the Escherichia coli expression vector pET11a, large amounts of a soluble but inactive form of the enzyme are produced upon its induction
-
cMDH cloned and overexpressed in Escherichia coli
-
cMDH is expressed in Escherichia coli M15 as a His-tagged fusion protein
-
cMDH, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, expression of N-terminal His-tagged cMDH in Escherichia coli strain BL21 (DE3) pLysS; expression in Escherichia coli
DNA and amino acid sequence determination and analysis, real-time quantitative RT-PCR expression analysis, recombinant ectopic expression in Solanum lycopersicum; gene MdcyMDH, DNA and amino acid sequence determination and analysis, genetic organization, expression analysis, overview. MdcyMDH gene, driven by theCaMV35S promoter, is introduced into tomato to evaluate the ex planta function of MdcyMDH ectopic expression on fruit acidity. MdcyMDH transcripts are detected in both fruits and functional leaves
Escherichia coli malate dehydrogenase gene cloned into expression vector pASK40 by PCR
expressed in Escherichia coli as inclusion bodies
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21pLys cells
-
expressed in Escherichia coli M15 (pREP4) cells
-
expressed in HEK-293 cells
-
expression in Escherichia coli
expression in Escherichia coli as a His-tagged protein
expression in Escherichia coli as protein fusion with glutathione S-transferase or a poly-His tail
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
from roots, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene cyMDH, expression in sense and antisense orientation in Solanum lycopersicum cv. Tianjinbaiguo leaves and Malus domesrtica callus tissue using the Agrobacterium strain LBA4404 transfection method, expression patterns analysis with real-time quantitative PCR, overview
gene encoding mitochondrial isoenzyme of MDH is present in a single copy in genomic DNA
-
gene encoding the halophilic MalDH cloned into M13mp18 phage
-
gene for tMDH expressed in Escherichia coli and sequence reported
-
gene mdh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3); gene mdh, DNA and amino acid sequence determination and analysis, phylogenetic tree
-
gene mdh, expression of the enzyme in strain BL21(DE3) as soluble protein
gene mdh, expression of wild-type and mutant enzymes in strains DH10B and W945T1-2
gene mdh, sequence comparisons and phylogenetic analysis, overview, overexpression of His6-tagged MDH in Escherichia coli strain Rosetta(DE3), subcloning in Escherichia coli strain DH5alpha
gene mmdh1, genotyping; gene mmdh2, genotyping
gene YlMDH1; gene YlMDH2, subject for alternative splicing, DNA and amino acid sequence determination and analysis, phylogenetic analysis, construction and generation of three different splicing variants of YlMDH2 and transcriptional regulation analysis, overview
genes pmdh1 and pmdh2
-
high throughput adult worm cDNA library screening, DNA and mino acid sequence determination and analysis, expression of the GST-tagged enzyme in Escherichia coli
mitochondrial and glycosomal malate dehydrogenase isoenzyme, functional expression in Escherichia coli
mitochondrial, glycosomal and cytosolic isoforms are expressed in Escherichia coli
-
overexpression in Escherichia coli
sequence comparisons, recombinant expression of His-tagged MDH in Escherichia coli strain Rosetta (DE3)
-
structural gene expressed in Escherichia coli expression vector pET11a, large amounts of soluble but inactive form of the enzyme produced upon its induction
-
two mMDHG cDNAs are cloned: FamMDH-I and Fa-mMDH-II
-
wild-type and E267R protein are overexpressed in Escherichia coli
-