1.1.1.36: acetoacetyl-CoA reductase

This is an abbreviated version, for detailed information about acetoacetyl-CoA reductase, go to the full flat file.

Reaction

(R)-3-hydroxyacyl-CoA
+
NADP+
=
3-oxoacyl-CoA
+
NADPH
+
H+

Synonyms

(3R)-hydroxyacyl-CoA dehydrogenase, (R)-3-hydroxyacyl-CoA dehydrogenase, acetoacetyl CoA reductase, acetoacetyl coenzyme A reductase, acetoacetyl-CoA reductase, beta-ketoacyl reductase, beta-ketoacyl-CoA reductase, beta-ketoacyl-coenzyme A reductase, D(-)-beta-hydroxybutyryl CoA-NADP oxidoreductase, D-3-hydroxyacyl-CoA dehydrogenase, D-3-hydroxyacyl-CoA reductase, D-specific 3-hydroxyacyl-CoA dehydrogenase, hydroxyacyl coenzyme-A dehydrogenase, KCR, MFE-2, More, NADP-linked acetoacetyl CoA reductase, NADPH-dependent acetoacetyl-CoA reductase, NADPH-linked acetoacetyl-CoA reductase, NADPH:acetoacetyl-CoA reductase, PHA-specific acetoacetyl-CoA reductase, PhaB, PhbB, polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase, polyhydroxybutyrate enzyme, short chain beta-ketoacetyl(acetoacetyl)-CoA reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.36 acetoacetyl-CoA reductase

Purification

Purification on EC 1.1.1.36 - acetoacetyl-CoA reductase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, DEAE-Sephasrose CL-6B, hydroxyapatite, Red-Sepharose CL-6B chromatography
-
ammonium sulfate fractionation, hydroxylapatite, Sephadex G-200 chromatography
-
ammonium sulfate fractionation, ion exchange, hydroxyapatite, affinity chromatography
-
Candida tropicalis recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] from Escherichia coli
-
DEAE-Sepharose chromatography, Blue gel column
-
fractionated with ammonium sulfate
Pigeon
-
gel-filtration Sephadex G-200, DEAE-cellulose chromatography, sucrose-gradient centrifugation
-
KCl extraction
-
NADH-linked: DEAE-Sepharose CL-6B, Blue Sepharose CL-6B, botyl Sepharose 4B, Mono Q 5/5; NADPH-linked: DEAE-Sepharose CL-6B, Red 120-agarose, phenyle Sepharose
-
PEG, DEAE-Toyopearl column
-
recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography
-
recombinant His-tagged, truncated enzyme from Escherichia coli, the His-tag is cleaved off by factor Xa
-
recombinant truncated enzyme from Escherichia coli, to homogeneity
-
Resource S cation exchange chromatography and Superdex 200 HR 10/30 column chromatography
-
the FOX2 gene is overexpressed from a multicopy vector (YEp352) in Saccharomyces cerevisiae and the gene product purified to apparent homogenity. A truncated version of MFP lacking 271 carboxyl-terminal amino acids is also overexpressed and purified
-