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1.1.1.36: acetoacetyl-CoA reductase

This is an abbreviated version!
For detailed information about acetoacetyl-CoA reductase, go to the full flat file.

Word Map on EC 1.1.1.36

Reaction

(R)-3-hydroxyacyl-CoA
+
NADP+
=
3-oxoacyl-CoA
 +
NADPH
 +
H+

Synonyms

(3R)-hydroxyacyl-CoA dehydrogenase, (R)-3-hydroxyacyl-CoA dehydrogenase, AAR, AcAc-CoA reductase, acetoacetyl CoA reductase, acetoacetyl coenzyme A reductase, acetoacetyl-CoA reductase, AKR1B15, aldo-keto reductase 1B15, beta-ketoacyl reductase, beta-ketoacyl-CoA reductase, beta-ketoacyl-coenzyme A reductase, D(-)-beta-hydroxybutyryl CoA-NADP oxidoreductase, D-3-hydroxyacyl-CoA dehydrogenase, D-3-hydroxyacyl-CoA reductase, D-specific 3-hydroxyacyl-CoA dehydrogenase, hydroxyacyl coenzyme-A dehydrogenase, KCR, ketoacyl reductase, MFE-2, More, MSMEG_6753, NADH-preferring acetoacetyl-CoA reductase, NADP-linked acetoacetyl CoA reductase, NADPH-dependent acetoacetyl coenzyme A reductase, NADPH-dependent acetoacetyl-CoA reductase, NADPH-dependent acetoacetyl-coenzyme A reductase, NADPH-linked acetoacetyl-CoA reductase, NADPH:acetoacetyl-CoA reductase, PHA-specific acetoacetyl-CoA reductase, PhaB, PhbB, polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase, polyhydroxybutyrate enzyme, short chain beta-ketoacetyl(acetoacetyl)-CoA reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.36 acetoacetyl-CoA reductase

Cloned

Cloned on EC 1.1.1.36 - acetoacetyl-CoA reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the yeast expression plasmid pYE352, Saccharomyces cerevisiae fox-2 cells, devoid of Sc MFE-2, transformed with pYE352::ScMFE-2(adelta) and pYE352::ScMFE-2(bdelta). The recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] transformed into Escherichia coli
-
DNA sequence determination and analysis, construction of 5 different expression constructs for transformation of Zea mays plants, one of which contains the small subunit 24 amino acid transit peptide of Pisum sativum ribulose bisphosphate carboxylase
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expressed in Brassica napus, transported with fusion peptide to seed leukoplast
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 Rosetta2 (DE3) pLysS cells and a fabG -temperature-sensitive mutant Escherichia coli CL104 strain
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli JM155
-
expressed in Escherichia coli strain JM109
-
expression of a truncated enzyme, comprising residues Met1-Asp319 of the dehydrogenase region, lacking the hydratase-2 region, in Escherichia coli
-
functional co-expression of PHA synthesis genes phaAB in Aeromonas hydrophila 4AK4 encoding beta-ketothiolase and the NADPH-dependent acetoacetyl-CoA reductase, leading to production of terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate), addition of propionate alters the monomer composition of the polymer, overview
-
gene AKR1B15 located on chromosome 7, cloning of two isoforms: AKR1B15.1 and AKR1B15.2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged isozymes in Escherichia coli strain BL21(DE3), recombinant expression of C- or N-terminally c-Myctagged isozymes in HEK-293 cells
gene fabG1, DNA sequence determination and analysis, expression in the fabG1-deficient mutant, phylogenetic tree, sequence comparison of FabG1 to FabG6
gene phaB, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene phaB, recombinant expression in Corynebacterium glutamicum strain ATCC 13803, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene phaB, recombinant expression of a PhaB mutant T173s enzyme from Ralstonia eutropha in Nicotiana tabacum cv. Bright Yellow-2 cells, the mutated PhaB increases the poly(3-hydroxybutyrate) (P(3HB)) content by threefold compared to control. Plasmids pRIphaC-phaB(WT) and pRIphaCphaB(TS) are introduced into BY-2 cells using the Agrobacterium-mediated transfection method. The PhaB-catalyzed reaction is suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells
gene phbB, functional expression in Escherichia coli strains BW25113 and DH5alpha, co-expression with genes phbA and tesB, encoding beta-ketothiolase and thioesterase II, respectively, to reconstruct the R-3-hydroxybutyric acid production pathway, optimization of culture conditions, overview
-
gene phbB, functional expression of the phaABCRe operon containing genes phbA, phbB and phbC of the PHA biosynthetic pathway of Ralstonia eutropha, which encode beta-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively, in Saccharomyces cerevisiae strain INVSc1/PHA1 and in non-convenient Kloeckera spp. strain KY1/PHA, reconstruction of the PHA biosynthetic pathway leading to accumulation of poly-3-hydroxybutyrate and copolymer poly-(3-hydroxybutyrate-co-poly-3-hydroxyvalerate), respectively, overview
-
isozymes KCR1 and KCR2, DNA and amino acid sequence determination, expression analysis, complementation of the yeast ybr159D mutant only isozyme KCR1, overview
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N-terminal part of amino acids 1-604 is expressed as a C-terminally His-tagged, stable protein in Escherichia coli strain BL21(DE3)
-
wild type (HsMFE-2) and its variants are expressed in Saccharomyces cerevisiae
-