1.1.1.345: D-2-hydroxyacid dehydrogenase (NAD+)
This is an abbreviated version!
For detailed information about D-2-hydroxyacid dehydrogenase (NAD+), go to the full flat file.
Reaction
Synonyms
D-2-HADH, D-2-hydroxyacid dehydrogenase, D-2-hydroxyisocaproate dehydrogenase, D-HicDH, D-isomer specific 2-hydroxyacid dehydrogenase, D-isomer specific NAD+-dependent 2-hydroxyacid dehydrogenase, D-isomer-specific 2-hydroxyacid dehydrogenase, D-lactate dehydrogenase, D-lactate:NAD+ oxidoreductase, D-malate dehydrogenases, D-mandelate dehydrogenase, D-ManDH1, D-ManDH2, D-MDH, D2-HDH, HdhD, Ldb1010, ldh1837, LEUM_1837, ManDH2, NAD-dependent D-2-hydroxyacid dehydrogenase, PanE, R-HicDH
ECTree
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Purification
Purification on EC 1.1.1.345 - D-2-hydroxyacid dehydrogenase (NAD+)
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DEAE-cellulose DE32 column chromatography, AMP-Sepharose column chromatography, and Mono Q column chromatography
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recombinant His-tagged D2-HDH from Escherichia coli BL21(DE3) pLysS by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Sepharose column chromatography, DEAE-cellulose column chromatography, Q-Sepharose column chromatography, Sephacryl S-300 gel filtration, and Sephadex G25 gel filtration
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