1.1.1.34: hydroxymethylglutaryl-CoA reductase (NADPH)

This is an abbreviated version, for detailed information about hydroxymethylglutaryl-CoA reductase (NADPH), go to the full flat file.

Reaction

(R)-mevalonate
+
CoA
+ 2 NADP+ =
(S)-3-hydroxy-3-methylglutaryl-CoA
+ 2 NADPH + 2 H+

Synonyms

3-hydroxy-3-methyl-glutaryl CoA reductase, 3-hydroxy-3-methylglutaryl Ccoenzyme A reductase 1, 3-hydroxy-3-methylglutaryl co-enzyme A reductase, 3-hydroxy-3-methylglutaryl CoA reductase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, 3-hydroxy-3-methylglutaryl coenzyme A reductase 1, 3-hydroxy-3-methylglutaryl-CoA reductase, 3-hydroxy-3-methylglutaryl-CoA reductase (NADPH), 3-hydroxy-3-methylglutaryl-coenzyme A reductase, 3-hydroxymethylglutaryl coenzyme A reductase, acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, beta-hydroxy-beta-methylglutaryl coenzyme A reductase, beta-hydroxy-beta-methylglutaryl-Co A reductase, EuHMGR, HMG CoA reductase, HMG-CoA reductase, HMG-CoAR, HMG1, HMG2, HMG2.2, Hmg2p, HMG3.3, HMGCoA reductase, HMGCoA reductase-mevalonate:NADP-oxidoreductase (acetylating CoA), HMGCoAR, HMGCR, HMGR, HMGR1, HMGR1S, HMGR2, hydroxy-3-methylglutoryl-Coenzyme A reductase, hydroxymethylglutaryl CoA reductase (NADPH), hydroxymethylglutaryl-coenzyme A reductase (reduced nicotinamide adenine dinucleotide phosphate), mevalonate:NADP+ oxidoreductase (acetylating CoA), microsomal HMG-CoA reductase, NADPH-hydroxymethylglutaryl-CoA reductase, S-3-hydroxy-3-methylglutaryl-CoA reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH)

Cloned

Cloned on EC 1.1.1.34 - hydroxymethylglutaryl-CoA reductase (NADPH)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA encoding the wild-type and mutant forms of human HMG-CoA reductase expressed under control of the yeast MET25 promoter in a Saccharomyces cerevisiae strain with deletions of both HMG1 and HMG2
-
coexpression of the Sulfolobus solfataricus hmgA gene in Escherichia coli with the argU gene that encodes tRNAAGA,AGG results in an over 10fold increase in enzyme yield; expression in Escherichia coli
-
construction of a N-terminally truncated enzyme, comprising the catalytic site, and subcloning into Escherichia coli strain DH5alpha
-
DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR and real-time PCR expression analysis
DNA and amino aid sequence determination and analysis, cloning in Escherichia coli and functional complementation of a HMGR-deficient mutant yeast strain using the yeast-Escherichia coli coli shuttle vector pYF1845
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3) as soluble protein
-
expression in Escherichia coli strain DH5alpha as His-tagged enzyme
-
expression of c-myc-tagged enzyme from plasmid in yeast, expression as GFP-fusion protein
-
expression of the His-tagged catalytic domain of the enzyme, residues E416-F887, in Escherichia coli
-
functional complementation of EuHMGR in HMGR-deficient mutant yeast JRY2394 demonstrates that EuHMGR mediates the mevalonate biosynthesis in yeast
gene hmgR, expression analysis under different salinity growth conditions, hmgR transcript increases in abundance with increasing salinity by a factor of 2.2fold
-
gene hmgr, genomic DNA sequence and full-length cDNA, as well as JcHMGR amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain Top 10F'
-
genes LcHMG1 and LcHMG2, DNA and amino acid sequence determination and analysis, real-time quantitative RT-PCR expression analysis
-
isozyme HMGR1, DNA and amino acid sequence determination and analysis, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system as His-tagged enzyme; isozyme HMGR2, DNA and amino acid sequence determination and analysis
overexpression in Escherichia coli
overexpression of the HMGR gene in transgenic Artemisia annua plants using Agrobacterium-mediated gene transfer technology
-
overexpression of the recombinant His-tagged enzyme in Lactococcus lactis strain NZ9000, co-expression with sesquiterpene synthase gene, PMSTS from Persicaria minor incorporated into the pNZ8048 plasmid, with two base substitutions, 796A.G and 1077A.G, introduced into the PMSTS gene during amplification resulting in exchange K266E and a silent mutation
-
the HMG1-overexpressing episome (pRS434GAP-HMG1) introduced into 36 type strains purchased from ATCC, and yeast recombinant AURGG101