1.1.1.319: isoeugenol synthase

This is an abbreviated version!
For detailed information about isoeugenol synthase, go to the full flat file.

Reaction

Synonyms

AIS1, CbIGS1, DcE(I)GS1, DcIGS1, eugenol/isoeugenol synthase, IGS, IGS1, isoeugenol synthase 1, More, PaAIS1, PhIGS1, t-anol/isoeugenol synthase 1

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.319 isoeugenol synthase

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Results	in table
7	AA Sequence
8	Cloned(Commentary)
8	Cofactor
7	General Information
7	Inhibitors
6	kcat/KM [mM/s]
6	KM Value [mM]
9	Natural Substrates/ Products (Substrates)
9	Organism
5	Pathway
5	pH Optimum
1	pH Range
11	Protein Variants
2	Purification (Commentary)
2	Reaction
9	Reference
14	Source Tissue
1	Specific Activity [micromol/min/mg]
15	Substrates and Products (Substrate)
18	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
3	Temperature Stability [°C]
6	Turnover Number [1/s]

Engineering

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Engineering on EC 1.1.1.319 - isoeugenol synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

L91I/L92H/D95N

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

R73K/V77I/S83P/V84F

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

R73K/V77I/S83P/V84F/Y87I/P88S/L91I/L92H/D95N

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

S83P/V84F

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

S83P/V84F/Y87I/P88S

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

V84F

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

V84F/Y87I

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

Y87I

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

Y87I/P88S

Clarkia breweri

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

V88F/Y91I

Petunia x hybrida

-

site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme

718214

additional information

Petunia x hybrida

Q15GI3

recombinant Petunia hybrida IGS expression alone does not increase the concentration of anol or isoeugenol in agroinfiltrated strawberry fruits, concurrent silencing of CHS substantially enhances the yield of the two metabolites

718078