1.1.1.31: 3-hydroxyisobutyrate dehydrogenase

This is an abbreviated version, for detailed information about 3-hydroxyisobutyrate dehydrogenase, go to the full flat file.

Reaction

3-hydroxy-2-methylpropanoate
+
NAD+
=
2-Methyl-3-oxopropanoate
+
NADH
+
H+

Synonyms

3-HIBADH, 3-hydroxyisobutyrate dehydrogenase, 3-hydroxyisobutyrate dehydrogenase-I, 3HIBDH-IV, 3HIBDHI, beta-hydroxyisobutyrate dehydrogenase, HIBADH, HIBDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.31 3-hydroxyisobutyrate dehydrogenase

Purification

Purification on EC 1.1.1.31 - 3-hydroxyisobutyrate dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
96fold purified at 4°C by ammonium sulfate fractionation and gel filtration; ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography; native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography
-
affinity chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
-
Ni-NTA resin column chromatography
-