1.1.1.302: 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase

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For detailed information about 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, go to the full flat file.

Reaction

2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one

2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one

Synonyms

2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase, 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase, arfC, MJ0671, MJ0671 pyrimidine reductase, MjaRED, ribD2

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.302 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase

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Results	in table
3247	AA Sequence
4	Cloned(Commentary)
6	Cofactor
1	Crystallization (Commentary)
6	General Information
7	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
9	Organism
6	Pathway
6	PDB ID
4	pH Optimum
3	Purification (Commentary)
2	Reaction
9	Reference
1	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
5	Subunits
15	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

Cloned

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Cloned on EC 1.1.1.302 - 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase

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expressed in Escherichia coli using a synthetic gene, the synthetic open reading frame is optimized for expression in Escherichia coli. The hypothetical open reading frame MJ0671 of Methanocaldococcus jannaschii predicts a protein of 224 amino acid residues. Because MJ0671 contains numerous codons that are poorly translated in Escherichia coli, a synthetic gene is designed that is optimized for the Escherichia coli codon usage. Approximately 31% (69 of 224) of the codons are replaced, and 14 singular restriction sites are introduced. The DNA sequence is assembled from 16 synthetic oligonucleotides by a sequence of eight PCR steps. The synthetic gene is transcribed efficiently in a recombinant Escherichia coli strain, affording approximately 30% of cellular protein

Methanocaldococcus jannaschii

700119

expression in Escherichia coli

2 entries

overexpressed in Escherichia coli

Methanocaldococcus jannaschii

698562