220.127.116.11: 3-hydroxybutyrate dehydrogenase
This is an abbreviated version, for detailed information about 3-hydroxybutyrate dehydrogenase, go to the full flat file.
3-D-hydroxybutyrate dehydrogenase, 3-HBDH, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase 2, 3-hydroxybutyrate dehydrogenase-2, 3HBDH, acetoacetyl-CoA reductase, BDH, BDH1, BDH2, BDH3, BdhA, beta-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, beta-hydroxybutyric dehydrogenase, D(-)-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, DHRS6, HBD, HBDH, hydroxybutyrate oxidoreductase, More, NAD-beta-hydroxybutyrate dehydrogenase
Storage Stability on EC 18.104.22.168 - 3-hydroxybutyrate dehydrogenase
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-20°C, 1 mg enzyme/ml, 0.2 mM potassium phosphate pH 8.0, 10% glycerol, 10 mM 2-mercaptoethanol, 6 months, 0% inactivation, 0°C, 0.1 mg enzyme/ml, 1 day, 50% inactivation
-20°C, 20 mM phosphate buffer, pH 7.0, 20% glycerol
-20°C, crude extract, partially purified enzyme, several weeks, 0% inactivation
-20°C, several months, 0%, 4°C, lyophilized, 2 month, 0% inactivation, 4°C, 0.1 M sodium phosphate buffer pH 7-8, 5 days, 50% inactivation, stability increases in the presence of 1-10 mM EDTA
4°C, Tris-HCl buffer, 50-100 mM Mg2+, Mn2+, Ba2+, or Ca2+, enzyme loses all activity in the presence of phosphate buffer or EDTA within 2 min