1.1.1.30: 3-hydroxybutyrate dehydrogenase

This is an abbreviated version, for detailed information about 3-hydroxybutyrate dehydrogenase, go to the full flat file.

Reaction

(R)-3-hydroxybutanoate
+
NAD+
=
acetoacetate
+
NADH
+
H+

Synonyms

3-D-hydroxybutyrate dehydrogenase, 3-HBDH, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase 2, 3-hydroxybutyrate dehydrogenase-2, 3HBDH, acetoacetyl-CoA reductase, BDH, BDH1, BDH2, BDH3, BdhA, beta-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, beta-hydroxybutyric dehydrogenase, D(-)-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, DHRS6, HBD, HBDH, hydroxybutyrate oxidoreductase, More, NAD-beta-hydroxybutyrate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.30 3-hydroxybutyrate dehydrogenase

Purification

Purification on EC 1.1.1.30 - 3-hydroxybutyrate dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
about 1000fold, to homogeneity from liver mitochondrial membranes by solubilization with Triton X-100, DEAE-ion-exchange chromatography and phenyl-resin chromatography
-
affinity chromatography on two triazine dyes
-
ammonium sulfate precipitation, DEAE-cellulose
-
ammonium sulfate precipitation, DEAE-Sephadex, Sephadex G-200
-
ammonium sulfate, acid precipitation, DEAE-Sepharose, glass beads
-
ammonium sulfate, DEAE-Sepharose, 60°C, 10 min, hydroxylapatite, octyl-Sepharose, Sephadex G-200, DEAE-Sepharose
-
BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography
charge-controlled hydrophobic chromatography and Sephadex G-100 gel filtration
-
DEAE-cellulose, Matrex gel blue A, Sephadex G-200, chromatofocusing
-
native by 3 chromatographic steps, recombinant from Escherichia coli in 2chromatographic steps
native enzyme by ammonium sulfate fractionation and affinity chromatography
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography, and TSK gel G3000SW gel filtration
-
phospholipase A2 treatment, controlled-pore glass beads
protamine sulfate precipitation, Sephacryl S-400, S200, DEAE-Sepharose
-
recombinant
-
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinant enzyme from Escherichia coli strain DH1 by anion exchange chromatography, hydrophobic interaction chromatography using a gradient of 1-20% ammonium sulfate, and ultrafiltration
-
wild-type and mutants purified by metal chelating affinity chromatography followed by ion-exchange chromatography
-