Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.1.30: 3-hydroxybutyrate dehydrogenase

This is an abbreviated version!
For detailed information about 3-hydroxybutyrate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.30

Reaction

(R)-3-hydroxybutanoate
+
NAD+
=
acetoacetate
+
NADH
+
H+

Synonyms

3-D-hydroxybutyrate dehydrogenase, 3-HBDH, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase 2, 3-hydroxybutyrate dehydrogenase-2, 3HBDH, acetoacetyl-CoA reductase, BDH, BDH1, BDH2, BDH3, BdhA, beta-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, beta-hydroxybutyric dehydrogenase, D(-)-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, DHRS6, HBD, HBDH, hydroxybutyrate oxidoreductase, More, NAD-beta-hydroxybutyrate dehydrogenase, Psyc_1428

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.30 3-hydroxybutyrate dehydrogenase

Purification

Purification on EC 1.1.1.30 - 3-hydroxybutyrate dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
about 1000fold, to homogeneity from liver mitochondrial membranes by solubilization with Triton X-100, DEAE-ion-exchange chromatography and phenyl-resin chromatography
-
affinity chromatography on two triazine dyes
-
ammonium sulfate precipitation, DEAE-cellulose
-
ammonium sulfate precipitation, DEAE-Sephadex, Sephadex G-200
-
ammonium sulfate, acid precipitation, DEAE-Sepharose, glass beads
-
ammonium sulfate, DEAE-Sepharose, 60°C, 10 min, hydroxylapatite, octyl-Sepharose, Sephadex G-200, DEAE-Sepharose
-
BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography
charge-controlled hydrophobic chromatography and Sephadex G-100 gel filtration
DEAE-cellulose, Matrex gel blue A, Sephadex G-200, chromatofocusing
-
native by 3 chromatographic steps, recombinant from Escherichia coli in 2chromatographic steps
native enzyme by ammonium sulfate fractionation and affinity chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography, and TSK gel G3000SW gel filtration
-
phospholipase A2 treatment, controlled-pore glass beads
protamine sulfate precipitation, Sephacryl S-400, S200, DEAE-Sepharose
-
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinant enzyme from Escherichia coli strain DH1 by anion exchange chromatography, hydrophobic interaction chromatography using a gradient of 1-20% ammonium sulfate, and ultrafiltration
wild-type and mutants purified by metal chelating affinity chromatography followed by ion-exchange chromatography