1.1.1.30: 3-hydroxybutyrate dehydrogenase
This is an abbreviated version!
For detailed information about 3-hydroxybutyrate dehydrogenase, go to the full flat file.
Word Map on EC 1.1.1.30
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1.1.1.30
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ketone
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succinate
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acetoacetate
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lipid-requiring
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lecithin
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coa-transferase
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acetoacetyl-coa
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alpha-glycerophosphate
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thiolase
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3-oxoacid
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submitochondrial
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ketogenesis
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enzyme-phospholipid
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analysis
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diagnostics
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synthesis
- 1.1.1.30
- ketone
- succinate
- acetoacetate
-
lipid-requiring
- lecithin
-
coa-transferase
- acetoacetyl-coa
- alpha-glycerophosphate
-
thiolase
-
3-oxoacid
-
submitochondrial
-
ketogenesis
-
enzyme-phospholipid
- analysis
- diagnostics
- synthesis
Reaction
Synonyms
3-D-hydroxybutyrate dehydrogenase, 3-HBDH, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase 2, 3-hydroxybutyrate dehydrogenase-2, 3HBDH, acetoacetyl-CoA reductase, BDH, BDH1, BDH2, BDH3, BdhA, beta-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, beta-hydroxybutyric dehydrogenase, D(-)-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, DHRS6, HBD, HBDH, hydroxybutyrate oxidoreductase, More, NAD-beta-hydroxybutyrate dehydrogenase, Psyc_1428
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Purification
Purification on EC 1.1.1.30 - 3-hydroxybutyrate dehydrogenase
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about 1000fold, to homogeneity from liver mitochondrial membranes by solubilization with Triton X-100, DEAE-ion-exchange chromatography and phenyl-resin chromatography
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ammonium sulfate, DEAE-Sepharose, 60°C, 10 min, hydroxylapatite, octyl-Sepharose, Sephadex G-200, DEAE-Sepharose
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BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography
charge-controlled hydrophobic chromatography and Sephadex G-100 gel filtration
DEAE-cellulose, Matrex gel blue A, Sephadex G-200, chromatofocusing
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native by 3 chromatographic steps, recombinant from Escherichia coli in 2chromatographic steps
native enzyme by ammonium sulfate fractionation and affinity chromatography
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phospholipase A2 treatment, controlled-pore glass beads
protamine sulfate precipitation, Sephacryl S-400, S200, DEAE-Sepharose
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recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
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recombinant enzyme from Escherichia coli strain DH1 by anion exchange chromatography, hydrophobic interaction chromatography using a gradient of 1-20% ammonium sulfate, and ultrafiltration
wild-type and mutants purified by metal chelating affinity chromatography followed by ion-exchange chromatography