1.1.1.30: 3-hydroxybutyrate dehydrogenase

This is an abbreviated version, for detailed information about 3-hydroxybutyrate dehydrogenase, go to the full flat file.

Reaction

(R)-3-hydroxybutanoate
+
NAD+
=
acetoacetate
+
NADH
+
H+

Synonyms

3-D-hydroxybutyrate dehydrogenase, 3-HBDH, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase 2, 3-hydroxybutyrate dehydrogenase-2, 3HBDH, acetoacetyl-CoA reductase, BDH, BDH1, BDH2, BDH3, BdhA, beta-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, beta-hydroxybutyric dehydrogenase, D(-)-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, DHRS6, HBD, HBDH, hydroxybutyrate oxidoreductase, More, NAD-beta-hydroxybutyrate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.30 3-hydroxybutyrate dehydrogenase

Inhibitors

Inhibitors on EC 1.1.1.30 - 3-hydroxybutyrate dehydrogenase

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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(DL)-2-hydroxybutyrate
-
5 mM, pH 8.5, 25°, 69% inhibition
(DL)-lactate
-
5 mM, pH 8.5, 25°C, 65% inhibition
2,4-Dichlorophenoxyacetic acid
-
inhibits the enzyme, in vivo treatment of the animals with 2,4-dichlorophenoxyacetic acid leads to strong decrease of triglycerides level and HDL cholesterol and an increase in GOT level and LDL cholesterol, and necrosis of seminiferous tubules cells in testis, hyperplasia of hepatocytes in liver and presence of multinucleated giant cells in brain, overview
2-Hydroxybutyrate
-
10 mM, 57% inhibition
2-oxobutyrate
-
10 mM, 29% inhibition
3-hydroxybutyrate
-
substrate inhibition at concentrations above 20 mM, 50% inhibition at 200 mM, cyanylated enzyme is 3fold more active at high substrate concentration
4-mercapto-3-nitrobenzoate
-
derivatization of enzyme sulfhydryl groups, less than 1% residual activity
5,5'-dithiobis(2-nitrobenzoic acid)
-
0.15 mM, 63% inhibition
acetoacetate
acetoacetyl-CoA
-
1 mM, 29% inhibition
acetyl-CoA
-
1 mM, 82% inhibition
acetylphosphonate
-
16.7 mM, pH 7, 28% inhibition
ADP
-
2.85 mM, 0.025 mM NADH, 63% inhibition, competitive inhibitor vs. NADH and NAD+, pH 7-7.5, noncompetitive vs. acetoacetate
ADP-ribose
-
competitive inhibition vs. coenzyme, noncompetitive vs. substrate
AMP
-
2.85 mM, 0.025 mM NADH, 55% inhibition, competitive inhibitor vs. NADH and NAD+, pH 7-7.5
ATP
-
2.85 mM, 0.025 mM NADH, 79% inhibition, competitive inhibitor vs. NADH and NAD+, pH 7-7.5, noncompetitive vs. acetoacetate
bromomalonate
Butanedione
-
-
Butyrate
cacodylate
-
competitive
chloromalonate
crotonate
-
10 mM, 20% inhibition
CuSO4
-
1 mM, 81% inhibition
D-lactate
-
5 mM, pH 8.5, 25°, 85% inhibition, uncompetitive vs. coenzyme, competitive vs. substrate
diazenedicarboxylic acid bis(dimethylamide)
dimethyl malonate
-
-
dimethyl phosphate
-
16.7 mM, pH 7, 12% inhibition
dimethylmalonate
dimethyloxyphosphinylacetate
-
competitive inhibitor vs. acetoacetate
DL-2-Hydroxybutyrate
-
-
DL-lactate
-
-
EDTA
inhibition and destabilization of the enzyme at 10 mM
glucose
inhibits the enzyme when contained in the growth medium
Hg2+
inhibition at 1 mM, and destabilization at 10 mM
HgCl2
Hydroxymalonate
KCl
-
80-100 mM, 50% inhibition
L-3-hydroxybutyrate
LiCl
-
80-100 mM, 50% inhibition
malonate
mesoxalate
methanephosphonic acid
-
3.3 mM, pH 7, 11% inhibition
methyl methylphosphonate
-
3.3 mM, pH 7, 13% inhibition
methyl phosphate
-
16.7 mM, pH 7, 21% inhibition
Methyl-2-methoxyphosphinylacetate
-
competitive inhibitor vs. acetoacetate
-
methylmalonate
MnCl2
-
1 mM, 50% inhibition
N-ethylmaleimide
NaCl
-
80-100 mM, 50% inhibition
NAD+
-
substrate inhibition, high concentrations, noncompetitive vs. beta-hydroxybutyrate
NH4Cl
-
80-100 mM, 50% inhibition
oxalate
-
16.7 mM, pH 7, 9% inhibition
p-chloromercuribenzoate
Phenylarsine oxide
-
NAD+ and NADH protect against inhibition
Phenylglyoxal
propionate
-
5 mM, pH 8.5, 25°C, 9% inhibition
pyruvate
sodium acetonylphosphonate
-
3.3 mM, pH 7, 21% inhibition
sodium monomethyl acetylphosphonate
-
16.7 mM, pH 7, 60% inhibition
sodium monomethylacetonylphosphate
-
16.7 mM, pH 7, 30% inhibition
Sodium sulfide
-
competitive vs. beta-hydroxybutyrate
sodium sulfite
-
uncompetitive vs. NAD+ and acetoacetate, competitive vs. beta-hydroxybutyrate
succinate
-
10 mM, 12% inhibition
Zn2+
inhibition and destabilization of the enzyme at 10 mM