1.1.1.3: homoserine dehydrogenase

This is an abbreviated version, for detailed information about homoserine dehydrogenase, go to the full flat file.

Reaction

L-homoserine
+
NAD(P)+
=
L-aspartate 4-semialdehyde
+
NAD(P)H
+
H+

Synonyms

AK-HDH, AK-HSD-1, AK-HSDH, aspartate kinase-homoserine dehydrogenase, aspartokinase-homoserine dehydrogenase I, HDH, hom-1, homoserine dehydrogenase 1, homoserine dehydrogenase 2, HSD, HSDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.3 homoserine dehydrogenase

Storage Stability

Storage Stability on EC 1.1.1.3 - homoserine dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.05 M potassium phosphate buffer, pH 7.5, 1.0 mM EDTA, 2.0 mM dithioerythritol, 5.0 mM L-threonine, 20% v/v glycerol, at least 2 years
-
-20°C, 30 mM potassium phosphate buffer, pH 7.5, 50% v/v glycerol, threonine resistant isozyme at least 2 months stable, threonine sensitive isozyme at least 4 months stable
-
-25°C, 50 mM potassium phosphate buffer, pH 7.2, 15% v/v glycerol, 1 mM EDTA, 1 mM threonine, 14 mM 2-mercaptoethanol
-
-80°C, purified recombinant soluble enzyme, at 3 mg/ml, stable