1.1.1.3: homoserine dehydrogenase

This is an abbreviated version, for detailed information about homoserine dehydrogenase, go to the full flat file.

Reaction

L-homoserine
+
NAD(P)+
=
L-aspartate 4-semialdehyde
+
NAD(P)H
+
H+

Synonyms

AK-HDH, AK-HSD-1, AK-HSDH, aspartate kinase-homoserine dehydrogenase, aspartokinase-homoserine dehydrogenase I, HDH, hom, hom-1, Hom6, homoserine dehydrogenase 1, homoserine dehydrogenase 2, HSD, HSDH, HseDH, orf19.2951, SACOL1362

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.3 homoserine dehydrogenase

Purification

Purification on EC 1.1.1.3 - homoserine dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme partially from seeds by ammonium sulfate fractionation and desalting gel filtration
-
overexpressed in Escherichia coli
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 70°C for 3 h, anion exchange chromatography, dialysis, and ultrafiltration
recombinant enzyme; recombinant enzyme; recombinant enzyme
recombinant His-tagged enzyme from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) codon plus-RIPL by heat treatment at 90°C for 10 min, nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration
recombinant separated catalytic domains
-
separation of the two isozymes using Blue-Sepharose chromatography
-
separation of the two isozymes using Matrex Gel Red A affinity chromatography
-
threonine sensitive and threonine insensitive isozymes
-