1.1.1.298: 3-hydroxypropionate dehydrogenase (NADP+)

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For detailed information about 3-hydroxypropionate dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

3-HIBADH, 3-hydroxyisobutyrate dehydrogenase, 3-hydroxypropionate dehydrogenase, bi-functional malonyl-CoA reductase, malonate semialdehyde reductase, malonate semialdehyde reductase (NADPH), malonic semialdehyde reductase, malonyl-CoA reductase, MCR, More, MSAR, Msed_1993

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.298 3-hydroxypropionate dehydrogenase (NADP+)

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Results	in table
2229	AA Sequence
3	Application
1	CAS Registry Number
8	Cloned(Commentary)
24	Cofactor
1	Crystallization (Commentary)
2	Expression
20	General Information
1	General Stability
4	Inhibitors
3	kcat/KM [mM/s]
11	KM Value [mM]
8	Metals/Ions
6	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
42	Organism
10	Pathway
6	pH Optimum
5	pH Range
9	Protein Variants
5	Purification (Commentary)
1	Reaction
20	Reference
3	Source Tissue
7	Specific Activity [micromol/min/mg]
1	Storage Stability
39	Substrates and Products (Substrate)
12	Subunits
40	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
4	Temperature Range [°C]
6	Temperature Stability [°C]
5	Turnover Number [1/s]

Cloned

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Cloned on EC 1.1.1.298 - 3-hydroxypropionate dehydrogenase (NADP+)

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli Rosetta 2(DE3)

Metallosphaera sedula

A4YI81

698628

expression in Escherichia coli strain BL21

Bacillus cereus

-

695710

gene mcr, functional expression in Escherichia coli under the T5 promoter control leading to production of 3-hydroxypropionate, coexpression with the nicotinamide nucleotide transhydrogenase that converts NADH to NADPH, required by MCR

Chloroflexus aurantiacus

Q6QQP7

725583

gene mcr, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of acetyl-CoA synthetase

Chloroflexus aurantiacus

Q6QQP7

762883

gene mcr, recombinant expression of MCR enzyme mutant in Escherichia coli strain CE43(DE3), coexpression with malonyl-CoA synthetase (MatB) from Rhodopseudomonas palustris, native transhydrogenase (PntAB), and NAD kinase (YfjB)

Chloroflexus aurantiacus

Q6QQP7

763124

gene mcr, recombinant expression of the Chloroflexus aurantiacus enzyme in Methylosinus trichosporium strain OB3b, subcloning in Escherichia coli strain DH5alpha

Chloroflexus aurantiacus

Q6QQP7

763426

gene mcr, separate recombinant expression of the N- and C-terminal subdomains of the enzyme, MCR-C and MCR-N, in Schizosaccharomyces pombe strain FY12804/NBRP, coexpression of acetyl-CoA synthase, gene acsSE, and aldehyde dehydrogenase, gene atd1, under the control of the cam1 promoter

Chloroflexus aurantiacus

Q6QQP7

763439

MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha

Bacillus cereus

-

695710