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1.1.1.282: quinate/shikimate dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about quinate/shikimate dehydrogenase [NAD(P)+], go to the full flat file.

Word Map on EC 1.1.1.282

Reaction

L-quinate
+
NAD(P)+
=
3-dehydroquinate
 +
NAD(P)H
 +
H+

Synonyms

cgl0424, cgR_0495, dehydroquinate dehydratase-shikimate dehydrogenase, More, NAD+ cofactor-specific QDH, NAD+-dependent enzyme quinate/shikimate dehydrogenase, NADP+ cofactor-specific QDH, NADP+-specific DHQD-QDH, PintaQDH, Poptr2, Poptr3, Poptr4, QDH, QSDH, qsuD, quinate dehydrogenase, quinate/shikimate 5-dehydrogenase, quinate/shikimate dehydrogenase, rifI, RifI2, SDH, SDH/QDH, sdhL, shikimate/quinate dehydrogenase, YdiB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.282 quinate/shikimate dehydrogenase [NAD(P)+]

Cloned

Cloned on EC 1.1.1.282 - quinate/shikimate dehydrogenase [NAD(P)+]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs)
gene cgR_0495, recombinant expression
gene expression analysis, phylogenetic analysis
gene PintaQDH, detailed phylogenetic analysis and tree
-
gene qsuD, recombinant expression
gene qsuD, recombinant expression in Escherichia coli
gene ydiB, recombinant overexpression in Escherichia coli strain PB12 causing a 500% increase in the molar yield of quinate and results in a 152% increase in quinate (mol/mol) relative to shikimate, with a sharp decrease in shikimate production. Transcriptomic analysis of PB12.SA22 and ydiB derivative strains
-
gene ydiB, the ydiB gene s cloned into plasmid pTOPO aroB aroE , resulting in the pTOPO ydiB aroB aroE derivative, enzyme overexpression in Escherichia coli strain PB12, quantitative RT-PCR analysis, coexpression of plasmid pTOPO aroB aroE and pJLB aroG fbr tktA and the cultivation of this derivative in Escherichia coli strain PB12.SA2 resulting in very high level expression of gene ydiB during exponential and stationary growth stages
-
recombinant expression of His6-tagged isozymes in Escherichia coli strain M15
-
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 gold
ydiB gene, expression in Escherichia coli BL21(DE3)