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1.1.1.270: 3beta-hydroxysteroid 3-dehydrogenase

This is an abbreviated version!
For detailed information about 3beta-hydroxysteroid 3-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.270

Reaction

a 3beta-hydroxysteroid
+
NADP+
=
a 3-oxosteroid
+
NADPH
+
H+

Synonyms

17beta-HSD7, 17beta-hydroxysteroid dehydrogenase type 7, 3-keto reducing enzyme, 3-keto reductase, 3-keto-reductase, 3-ketoreductase, 3-ketosteroid reductase, 3-KSR, 3-oxo steroid reductase, 3-oxosteroid reductase, 3beta-HSD, 3beta-HSD1, 3beta-HSD2, 3beta-HSDH, 3beta-hydroxysteroid dehydrogenase, 3beta-hydroxysteroid dehydrogenase type 1, 3beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type 1, 3beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type 2, 3beta-hydroxysteroid dehydrogenase/isomerase, AKR1C1, AKR1C2, AKR1Cs, aldo-keto reductase 1C2, CDD59473, Erg27, Erg27p, Hsd17, HSD17B7, HSD3B1, reductase, 3-oxo steroid, reductive 3beta-HSD, SDR11E1, SDR11E2, steroid-3-ketoreductase, sterol 3-ketoreductase, sterone-reducing enzyme, type 3 3alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.270 3beta-hydroxysteroid 3-dehydrogenase

Engineering

Engineering on EC 1.1.1.270 - 3beta-hydroxysteroid 3-dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D61N
-
site-directed mutagenesis, mutation in isozyme 3beta-HSD1
D61V
-
site-directed mutagenesis, mutation in isozyme 3beta-HSD1
DELTA214-341
-
inactive mutant enzyme
E192A
-
site-directed mutagenesis, mutation in isozyme 3beta-HSD1
P195R
-
site-directed mutagenesis, mutation in isozyme 3beta-HSD2
R195P
S194G
trilostane inhibits chimeric S194G-1 mutant (Ki: 0.00067 mM) with a noncompetitive mode compared to its 6.7fold higher affinity, competitive inhibition of 3beta-HSD1. 2,16-Dicyano-4,5-epoxy-androstane-3,17-dione inhibits S194G-1 with a 6.3fold higher Ki (0.0295 mM) than measured for 3beta-HSD1 (Ki: 0.0047 mM) but with the same competitive mode for both enzyme species
T8A
-
site-directed mutagenesis, mutation in isozyme 3beta-HSD1
F303Q
-
site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme
F303Q/M304S
-
site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme. The double mutation impairs the affinity and catalytic efficiency, although it did not affect the stereospecific reduction of the two 3-ketosteroids into the corresponding 3beta-hydroxysteroids
L116F
-
site-directed mutagenesis
M304S
-
site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme. The M304S mutation causes a 4fold increase in the Km value for pyridine-3-aldehyde
K206A
strain can be used to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially
R40A
strain can be used as a source of 4-methylzymosterone
additional information