1.1.1.26: glyoxylate reductase

This is an abbreviated version, for detailed information about glyoxylate reductase, go to the full flat file.

Reaction

glycolate
+
NAD+
=
glyoxylate
+
NADH
+
H+

Synonyms

GHPR, glycolate oxidase, glyoxylate reductase isoform 1, glyoxylate/hydroxypyruvate reductase, glyoxylic acid reductase, GLYR1, GLYR2, GxrA, NADH-dependent glyoxylate reductase, NADH-glyoxylate reductase, reductase, glyoxylate, SSO3187, succinic semialdehyde/glyoxylate reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.26 glyoxylate reductase

Cloned

Cloned on EC 1.1.1.26 - glyoxylate reductase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells
expression in Epicurean coli BL21 (DE3) as fusion protein from a His-tag plasmid
-
expression in Escherichia coli
expression of His6-tagged enzyme in Escherichia coli srain BL21
-
gene gxrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21
-