1.1.1.25: shikimate dehydrogenase

This is an abbreviated version, for detailed information about shikimate dehydrogenase, go to the full flat file.

Reaction

shikimate
+
NADP+
=
3-dehydroshikimate
+
NADPH
+
H+

Synonyms

3-dehydroquinate dehydratase/shikimate dehydrogenase, 5-dehydroshikimate reductase, ael1, Af2327, AroE, cgR_0495, cgR_1677, dehydroquinate dehydratase-shikimate dehydrogenase, dehydroshikimic reductase, DHD/SHD, DHQ-SDH, HI0607, More, MtbSD, NADP-dependent shikimate dehydrogenase, qsuD, rifI, SD, SDH, sdhL, shikimate 5-dehydrogenase, shikimate dehydrogenase, shikimate oxidoreductase, shikimate:NADP oxidoreductase, shikimate:NADP+ 5-oxidoreductase, SKDH, tm0346

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.25 shikimate dehydrogenase

Cloned

Cloned on EC 1.1.1.25 - shikimate dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis; DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants; expression in Escherichia coli; expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli in native and His-tagged form
expression in Escherichia coli; expression in Escherichia coli
expression in Escherichia coli; overexpression in Escherichia coli
expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain DL41
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs); five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs)
gene aroE, DNA and amino acid sequence determination, functional expression as soluble enzyme in Escherichia coli strain BL21(DE3)
gene aroE, expression of GST-tagged SeMet-labeled enzyme in Escherichia coli strain BL21
gene aroE, expression of His-tagged AroE in Escherichia coli strain BL21(DE3)
-
gene aroE, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene aroE, overexpression in the aroE auxotrophic mutant strain AB2834; overexpression in Escherichia coli K12
-
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
phylogenetic analysis of the three paralogues YdiB, AroE, and HI0607, DNA and amino acid sequence determination and analysis of paralogue HI0607, expression of wild-type paralogue HI0607 in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in strain B834(DE3)
-
plasmid pET23a(+)::aroE (either wild-type or mutants) transformed into Escherichia coli C41(DE3)
-
SDH-encoding gene is amplified by polymerase chain reaction from Pseudomonas putida KT2440 genomic DNA using primers with incorporated restriction sites. Resulting product is cloned into a vector and expressed in the Escherichia coli strain BL21 with appropriate antibiotics.; SDH-encoding gene is amplified by polymerase chain reaction from Pseudomonas putida KT2440 genomic DNA using primers with incorporated restriction sites. Resulting product is cloned into a vector and expressed in the Escherichia coli strain BL21 with appropriate antibiotics.; SDH-encoding gene is amplified by polymerase chain reaction from Pseudomonas putida KT2440 genomic DNA using primers with incorporated restriction sites. Resulting product is cloned into vector and expressed in the Escherichia coli strain BL21 with appropriate antibiotics.; SDH-encoding gene is amplified by polymerase chain reaction from Pseudomonas putida KT2440 genomic DNA using primers with incorporated restriction sites. Resulting product is cloned into vector and expressed in the Escherichia coli strain BL21 with appropriate antibiotics.; SDH-encoding gene is amplified by polymerase chain reaction from Pseudomonas putida KT2440 genomic DNA using primers with incorporated restriction sites. Resulting product is cloned into vector and expressed in the Escherichia coli strain BL21 with appropriate antibiotics.
wild-type and mutant cloned into vector pET22b and transformed into Escherichia coli strain BL21(DE3). Selenomethionine-substituted SDH generated in the methionine auxotrophic Escherichia coli strain B834(DE3)