1.1.1.248: salutaridine reductase (NADPH)

This is an abbreviated version, for detailed information about salutaridine reductase (NADPH), go to the full flat file.

Reaction

salutaridinol
+
NADP+
=
salutaridine
+
NADPH
+
H+

Synonyms

More, reductase, salutaridine 7-, SalR, salutaridine 7-reductase, salutaridine reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.248 salutaridine reductase (NADPH)

Crystallization

Crystallization on EC 1.1.1.248 - salutaridine reductase (NADPH)

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
construction of a homology model of the Papaver bracteatum SalR based on the X-ray structure of human carbonyl reductase 1, overview
-
purified recombinant detagged wild-type and selenomethionine-substituted SalR, hanging drop vapour diffsion method, mixing of 0.002 ml of 6 mg/ml protein in 20 mM Tris buffer, pH 7.5, containing 150 mM NaCl, 5 mM 2-mercaptoethanol, and 4 mM NADPH, with 0.002 ml of reservoir solution containing 0.1 M MES, pH 6.0-6.6, 1.9 M ammonium sulfate, 5% v/v PEG 400, 0.1 M LiCl, and 3% v/v glycerol, 3 weeks, 4°C, X-ray diffraction structure determination and analysis at 1.9 A resolution
to 1.9 A resolution, space group P6422 or P6222. Presence of NADPH is required for crystallization
-