1.1.1.244: methanol dehydrogenase

This is an abbreviated version, for detailed information about methanol dehydrogenase, go to the full flat file.

Reaction

methanol
+
NAD+
=
formaldehyde
+
NADH
+
H+

Synonyms

dehydrogenase, methanol, EC 1.1.99.8, MDH, MEDH, More, mxaF, NAD+ dependent methanol dehydrogenase, XoxF

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.244 methanol dehydrogenase

Engineering

Engineering on EC 1.1.1.244 - methanol dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D100N
-
strongly reduced NAD+-binding, no activity
D88N
-
only minor effects on activity
G13A
-
only minor effects on activity
G15A
-
only minor effects on activity
G95A
-
impaired cofactor binding, low acitivity
K103R
-
strongly reduced NAD+-binding, no activity
S97G
-
impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein
S97T
-
impaired cofactor binding, much higher acitivity than the wild type enzyme, no activation with ACT protein