220.127.116.11: 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)
This is an abbreviated version, for detailed information about 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+), go to the full flat file.
17-ketoreductase, 17beta-HSD, 17beta-hydroxysteroid dehydrogenase type 5, 3alpha(17beta)-HSD, AKR1C3, aldo-keto reductase 1C3, dehydrogenase, 3alpha,17beta-hydroxy steroid, type 2 3alpha-hxdroxysteroid dehydrogenase/type 5 17beta-hydroxysteroid dehydrogenase, type 2 3alppha-hydroxysteroid dehydrogenase/type 5 17beta-hydroxysteroid dehydrogenase, type 3 17beta-hydroxysteroid dehydrogenase, type 5 beta-hydroxysteroid dehydrogenase
Storage Stability on EC 18.104.22.168 - 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)
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-20°C, 20 mM potassium phosphate, 50% glycerol, 1 mM dithiotreitol, 1 mM EDTA, pH 7.5, 1 year, no loss in activity
-80°C, 30 mM Tris-HCl, pH 8.4, 1 mM EDTA, 1 mM dithiothreitol, several months, no loss of activity
4°C, 10 mM Tris/HCl buffer, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA
4°C, 50 mM Tris-HCl, pH 9.0, 20% glycerol, 100 mM KCl, 5 mg/ml N-octyl glucoside, 24 h, 20% loss of activity, -20°C, 1 week, no loss of activity
4°C-6°C, 60 mM sodium diphosphate, pH 9.5, a few days, no loss in activity