1.1.1.237: hydroxyphenylpyruvate reductase

This is an abbreviated version, for detailed information about hydroxyphenylpyruvate reductase, go to the full flat file.

Reaction

3-(4-hydroxyphenyl)lactate
+
NAD+
=
3-(4-hydroxyphenyl)pyruvate
+
NADH
+
H+

Synonyms

4-hydroxyphenylpyruvate reductase, H(P)PR, HPPR, HPRP, hydroxy(phenyl)pyruvate reductase, hydroxyphenylpyruvate reductase, hydroxyphenylpyruvic acid reductase, Ppr, pprA

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.237 hydroxyphenylpyruvate reductase

Crystallization

Crystallization on EC 1.1.1.237 - hydroxyphenylpyruvate reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
apoenzyme and enzyme in complex with NADP+, sitting drop vapour diffusion method, mixing of 0.001 ml of 6 mg/ml protein or 15 mg/ml protein with 2 mM NADP+ in 50 mM Tris-HCl, pH 7.0, with 0.001 ml of well solution containing 30% 2-methyl-2,4-pentanediol, 0.2 M NaCl, 0.1 M Tris-HCl, pH 7.5 at 4°C, or 20% PEG 1000, 0.1 M imidazole, pH 7.5, 4 mM 4-hydroxyphenylpyruvate, 0.2 mM DTT at 26°C, leading to one-crystal clusters or tetragonal bipyramid-shaped crystals, X-ray diffraction structure determination and analysis at 1.47 A and 2.2 A, respectively, modeling