1.1.1.23: histidinol dehydrogenase

This is an abbreviated version, for detailed information about histidinol dehydrogenase, go to the full flat file.

Reaction

L-Histidinol
+ 2 NAD+ +
H2O
=
L-histidine
+ 2 NADH + 3 H+

Synonyms

BN980_GECA03s06082g, BsHDH, GcHDH, HDH, HIS4 protein, HisD, histidinol dehydrogenase, HLDase, L-histidinol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.23 histidinol dehydrogenase

Purification

Purification on EC 1.1.1.23 - histidinol dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
55C, 40% ammonium sulfate, Sephadex G-50, DEAE-cellulose, crystallization
60C, pH 5 treatment, ammonium sulfate precipitation, gel filtration, DEAE-cellulose, gel filtration
-
ammonium sulfate precipitation, DEAE-chromatography, L-histidinol-Sepharose 4B affinity chromatography
-
ammonium sulfate precipitation, heating, affinity chromatography, preparative gel
-
enzyme expressed in SF9 cells, cell homogenate, Mono Q FPLC
-
heating, pH 4.8, ammonium sulfate, DEAE-cellulose
-
recombinant enzyme by anion exchange chromatography and gel filtration
-
recombinant enzyme from Escherichia coli strain BL21(DE3) 40fold to homogeneity by anion exchange chromatography, ultrafiltration, gel filtration, and another step of anion exchange chromatography
I6Y6Z1
recombinant enzyme fusion protein
-
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography, tag cleavage, and gel filtration, and recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
-
recombinant protein
-