1.1.1.23: histidinol dehydrogenase

This is an abbreviated version, for detailed information about histidinol dehydrogenase, go to the full flat file.

Reaction

L-Histidinol
+ 2 NAD+ +
H2O
=
L-histidine
+ 2 NADH + 3 H+

Synonyms

GcHDH, HDH, HIS4 protein, HisD, histidinol dehydrogenase, HLDase, L-histidinol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.23 histidinol dehydrogenase

Purification

Purification on EC 1.1.1.23 - histidinol dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
55C, 40% ammonium sulfate, Sephadex G-50, DEAE-cellulose, crystallization
60C, pH 5 treatment, ammonium sulfate precipitation, gel filtration, DEAE-cellulose, gel filtration
-
ammonium sulfate precipitation, DEAE-chromatography, L-histidinol-Sepharose 4B affinity chromatography
-
ammonium sulfate precipitation, heating, affinity chromatography, preparative gel
-
enzyme expressed in SF9 cells, cell homogenate, Mono Q FPLC
-
heating, pH 4.8, ammonium sulfate, DEAE-cellulose
-
recombinant enzyme from Escherichia coli strain BL21(DE3) 40fold to homogeneity by anion exchange chromatography, ultrafiltration, gel filtration, and another step of anion exchange chromatography
I6Y6Z1
recombinant enzyme fusion protein
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
-
recombinant protein
-