1.1.1.23: histidinol dehydrogenase
This is an abbreviated version!
For detailed information about histidinol dehydrogenase, go to the full flat file.
Reaction
Synonyms
11412251, BN980_GECA03s06082g, BsHDH, GcHDH, HDH, HIS4 protein, HisD, histidinol dehydrogenase, HLDase, L-histidinol dehydrogenase
ECTree
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Purification
Purification on EC 1.1.1.23 - histidinol dehydrogenase
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55°C, 40% ammonium sulfate, Sephadex G-50, DEAE-cellulose, crystallization
60°C, pH 5 treatment, ammonium sulfate precipitation, gel filtration, DEAE-cellulose, gel filtration
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ammonium sulfate precipitation, DEAE-chromatography, L-histidinol-Sepharose 4B affinity chromatography
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ammonium sulfate precipitation, heating, affinity chromatography, preparative gel
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heating, pH 4.8, ammonium sulfate, DEAE-cellulose
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recombinant enzyme by anion exchange chromatography and gel filtration
recombinant enzyme from Escherichia coli strain BL21(DE3) 40fold to homogeneity by anion exchange chromatography, ultrafiltration, gel filtration, and another step of anion exchange chromatography
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography, tag cleavage, and gel filtration, and recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
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55°C, 40% ammonium sulfate, Sephadex G-50, DEAE-cellulose, crystallization
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