1.1.1.22: UDP-glucose 6-dehydrogenase

This is an abbreviated version, for detailed information about UDP-glucose 6-dehydrogenase, go to the full flat file.

Reaction

UDP-alpha-D-glucose
+ 2 NAD+ +
H2O
=
UDP-alpha-D-glucuronate
+ 2 NADH + 2 H+

Synonyms

ADH-like UDP-glucose dehydrogenase, AglM, BceC, dehydrogenase, uridine diphosphoglucose, dual specificity UDP-glucose dehydrogenase, GbUGD, HasB, HasB2, HVO_1531, PA2022, PA3559, sqv-4, Sugarless protein, UDG1, UDP-alpha-D-glucose:NAD oxidoreductase, UDP-D-glucose dehydrogenase, UDP-GDH, UDP-Glc dehydrogenase, UDP-Glc DH, UDP-GlcDH, UDP-GlcDHase, UDP-glucose dehydrogenase, UDPDH, UDPG dehydrogenase, UDPG-DH, UDPG:NAD oxidoreductase, UDPGD, UDPGDH, UDPGDH-A, UDPGDH-B, UDPGlc dehydrogenase, UDPglucose dehydrogenase, UDPglucose:NAD+ oxidoreductase, Ugd, UGD1, UgdG, UGDH, uridine diphosphate D-glucose dehydrogenase, uridine diphosphate glucose dehydrogenase, uridine diphosphate glucose-6-dehydrogenase, uridine diphosphate-glucose dehydrogenase, uridine diphosphoglucose dehydrogenase, uridine-5'-diphosphoglucose dehydrogenase, uridyl phosphate dehydrogenase, VNG1048G, VNG_1048G

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.22 UDP-glucose 6-dehydrogenase

Cloned

Cloned on EC 1.1.1.22 - UDP-glucose 6-dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens
expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli
expression in Pichia pastoris
expression of isoforms Ugd2, Ugd3, Ugd4 in Escherichia coli
-
expression of mutant enzyme wild-type enzymes in Escherichia coli
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gene aglM, recombinant expression of CBD-tagged and C-terminally poly-His-tagged enzyme
-
gene bceC, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
gene udg1, recombinant expression of CBD-tagged and C-terminally poly-His-tagged enzyme
gene Ugd(BCAL2946) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAL2946) expressed in Escherichia coli HMS 174(DE3); gene Ugd(BCAM0855) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM0855) expressed in Escherichia coli HMS 174(DE3); gene Ugd(BCAM2034) cloned into the pGEM-T Easy vector, sequenced, and ligated into pET29a vector to transform Escherichia coli Top10 cells. Ugd(BCAM2034) expressed in Escherichia coli BL21 (DE3)
B4EB36, B4EMQ8, B4EN77
gene ugd, located near the colanic acid/group 1 CPS locus, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain CWG876; gene ugd, located near the colanic acid/group 1 CPS locus, recombinant overexpression of His6-tagged full-length enzyme and truncated enzyme comprising residues 447-704 in Escherichia coli strain CWG876
-
gene ugdG, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH6alpha
gene ugdG, expression of His6-tagged wild-type and selenomethionine-labeled UgdG in Escherichia coli BL21 and B843, respectively
gene UgdG, overexpression of His6-tagged enzyme in Escherichia coli
gene UGDH, real-time quantitative PCR enzyme expression analysis
genes szHasA (hyaluronan synthase gene) and szHasB (UDP-glucose-6-dehydrogenase gene) introduced into Lactococcus lactis strain NZ9000 under the control of nisA promoter and lacA promoter respectively, resulting in a dual-plasmid controlled expression system
-
hUGDH gene is cloned from a LNCaP cDNA library, from LNCaP C33 and C81 cells, expression of His6-tagged UGDH in Escherichia coli, expression of UGDH mutant D280N in HEK.293 cells
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overexpression as glutathione-S-transferase fusion protein in Escherichia coli
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overexpression in Escherichia coli JM109
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overexpression of the enzyme from Xenopus laevis in human smooth muscle cells increases the accumulation of hyaluronan
recombinant enzyme overexpression
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recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta2(DE3)pLysS, recombinant expression in HEK293 cells
-
recombinant UGDH expressed in Escherichia coli
-
the enzyme UGDH is encoded by two paralogous genes, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, cloning in a plasmid with the CaMV 35S promoter, the GUS gene, and the nopaline synthase terminator, recombinant overexpression of UDP-glucose dehydrogenase from Larix gmelinii in Arabidopsis thaliana enhancing the content of soluble sugars and hemicelluloses and growth and cold tolerance in the transgenic plants, transfection via Agrobacterium tumefaciens strain LBA4404, realtime and reverse transcriptase PCR expression analysis. The transgenic plants show distinct phenotypes at different developmental stages, as compared to the wild-type, overview